7-Deaza-2′-deoxy-guanosine-5′-triphosphate
ROCHE/10988537001 - 95% (7-Deaza-dGTP, HPLC), ≤4% (7-Deaza-dGDP, HPLC), solution, 10 mM
Synonym: 7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt; −N7-dGTP; 7-Deaza-dGTP
CAS Number: 101515-08-6
Empirical Formula (Hill Notation): C11H17N4O13P3
Molecular Weight: 506.19
MDL Number: MFCD00171614
Linear Formula: C11H17N4O13P3
Product Type: Chemical
composition | 7-Deaza-dGDP, ≤4% HPLC |
7-Deaza-dGTP, 95% HPLC | |
concentration | 10 mM |
form | solution |
InChI | 1S/C11H17N4O13P3/c12-11-1 |
InChI key | DLLXAZJTLIUPAI-UHFFFAOYSA |
manufacturer/tradename | Roche |
packaging | pkg of 200 μL (10 mM, 2 μmol) |
Quality Level | 100 ![]() |
SMILES string | NC1=NC(=O)c2ccn(C3CC(O)C( |
storage temp. | −20°C |
Analysis Note: | Absorption: 25 A260 units of product correspond to 1 mg. |
Analysis Note: | Absorbance determination: A250/A260, A280/A260, A290/A260 |
Application: | 7-Deaza-dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase. 7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA. Partial substitution of 7-Deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures). |
General description: | 7-deaza-2′-deoxy-guanosin |
General description: | The product contains 7-Deaza-2′-deoxy-guanosin |
Other Notes: | When PCR products contain 7-Deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished. PCR amplification efficiency is generally not affected by 7-Deaza-dGTP. For life science research only. Not for use in diagnostic procedures. |
Preparation Note: | Working solution: Dilute 7-deaza-dGTP into a solution containing 50 mM Tris, pH 7.0, and use at exactly the same concentration as you would use dGTP. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | No data available |
Flash Point(C) | No data available |
Storage Temp. | −20°C |
UNSPSC | 41116100 |