Advanced Search



7-Deaza-2′-deoxy-guanosine-5′-triphosphate

ROCHE/10988537001 - 95% (7-Deaza-dGTP, HPLC), ≤4% (7-Deaza-dGDP, HPLC), solution, 10 mM

Synonym: 7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt; −N7-dGTP; 7-Deaza-dGTP

CAS Number: 101515-08-6
Empirical Formula (Hill Notation): C11H17N4O13P3
Molecular Weight: 506.19
MDL Number: MFCD00171614
Linear Formula: C11H17N4O13P3
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-10988537001 200 µL
$232.00
1/EA
Add To Favorites

 

composition 7-Deaza-dGDP, ≤4% HPLC
  7-Deaza-dGTP, 95% HPLC
concentration 10 mM
form solution
InChI 1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)
InChI key DLLXAZJTLIUPAI-UHFFFAOYSA-N
manufacturer/tradename Roche
packaging pkg of 200 μL (10 mM, 2 μmol)
Quality Level 100 
SMILES string NC1=NC(=O)c2ccn(C3CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)c2N1
storage temp. −20°C
Analysis Note: Absorption: 25 A260 units of product correspond to 1 mg.
Analysis Note: Absorbance determination: A250/A260, A280/A260, A290/A260
Application: 7-Deaza-dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.
Partial substitution of 7-Deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures).
General description: 7-deaza-2′-deoxy-guanosine-5′-triphosphate is a nucleotide analog that is a candidate substrate for processive telomerase. The use of this analog improves PCR (polymerase chain reaction) yields. This compound also makes sequencing of GC rich sequences easier, especially if template amount is low and/or DNA quality is not good.
General description: The product contains 7-Deaza-2′-deoxy-guanosine-5′-triphosphate as a 10 mM solution of the lithium salt.
Other Notes: When PCR products contain 7-Deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished.
PCR amplification efficiency is generally not affected by 7-Deaza-dGTP.
For life science research only. Not for use in diagnostic procedures.
Preparation Note: Working solution: Dilute 7-deaza-dGTP into a solution containing 50 mM Tris, pH 7.0, and use at exactly the same concentration as you would use dGTP.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) No data available
Flash Point(C) No data available
Storage Temp. −20°C
UNSPSC 41116100

The following items have been added to your cart:

Choose a favorite list for this item:

Catalog Number Description Price
$

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.


Quick Order

* Required


New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.

800-334-7725
office@krackeler.com


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top