7-Deaza-2′-deoxy-guanosine-5′-triphosphate

ROCHE/10988537001 - 95% (7-Deaza-dGTP, HPLC), ≤4% (7-Deaza-dGDP, HPLC), solution, 10 mM

Synonym: 7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt; −N⁷-dGTP; 7-Deaza-dGTP

CAS Number: 101515-08-6
Empirical Formula (Hill Notation): C₁₁H₁₇N₄O₁₃P₃
Molecular Weight: 506.19
MDL Number: MFCD00171614
Linear Formula: C₁₁H₁₇N₄O₁₃P₃
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-10988537001	200 µL	$232.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≤4% (7-Deaza-dGDP, HPLC)
	95% (7-Deaza-dGTP, HPLC)
concentration	10 mM
form	solution
InChI	1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)
InChI key	DLLXAZJTLIUPAI-UHFFFAOYSA-N
manufacturer/tradename	Roche
packaging	pkg of 200 μL (10 mM, 2 μmol)
Quality Level	100
SMILES string	NC1=NC(=O)c2ccn(C3CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)c2N1
storage temp.	−20°C

Analysis Note:	Absorption: 25 A₂₆₀ units of product correspond to 1 mg.
Analysis Note:	Absorbance determination: A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀
Application:	7-Deaza-dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase. 7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA. Partial substitution of 7-Deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures).
General description:	7-deaza-2′-deoxy-guanosine-5′-triphosphate is a nucleotide analog that is a candidate substrate for processive telomerase. The use of this analog improves PCR (polymerase chain reaction) yields. This compound also makes sequencing of GC rich sequences easier, especially if template amount is low and/or DNA quality is not good.
General description:	The product contains 7-Deaza-2′-deoxy-guanosine-5′-triphosphate as a 10 mM solution of the lithium salt.
Other Notes:	When PCR products contain 7-Deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished. PCR amplification efficiency is generally not affected by 7-Deaza-dGTP. For life science research only. Not for use in diagnostic procedures.
Preparation Note:	Working solution: Dilute 7-deaza-dGTP into a solution containing 50 mM Tris, pH 7.0, and use at exactly the same concentration as you would use dGTP.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	No data available
Flash Point(C)	No data available

Purity	≤4% (7-Deaza-dGDP, HPLC); 95% (7-Deaza-dGTP, HPLC)
Storage Temp.	−20°C
UNSPSC	41116100

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