Antipain dihydrochloride

ROCHE/11004646001 - crystalline, suitable for cell culture, pkg of 10 mg

Synonym: Antipain dihydrochloride from microbial source; N-(Nα-Carbonyl-Arg-Val-Arg-al)-Phe

CAS Number: 37682-72-7
Empirical Formula (Hill Notation): C₂₇H₄₄N₁₀O₆ · 2 HCl
Molecular Weight: 677.62
MDL Number: MFCD00135957
Linear Formula: C₂₇H₄₄N₁₀O₆ · 2 HCl
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-11004646001	10 mg	$240.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

form	crystalline
InChI	1S/C27H44N10O6.2ClH/c1-16(2)21(23(40)34-18(15-38)10-6-12-32-25(28)29)37-22(39)19(11-7-13-33-26(30)31)35-27(43)36-20(24(41)42)14-17-8-4-3-5-9-17;;/h3-5,8-9,15-16,18-21H,6-7,10-14H2,1-2H3,(H,34,40)(H,37,39)(H,41,42)(H4,28,29,32)(H4,30,31,33)(H2,35,36,43);2*1H/t18?,19-,20?,21-;;/m0../s1
InChI key	YAHXZYICKJUJEO-BXLPLHKWSA-N
manufacturer/tradename	Roche
mol wt	M_r = 677.63
mp	209-217 °C
packaging	pkg of 10 mg
Quality Level	100
SMILES string	Cl[H].Cl[H].[H]C(=O)C(CCCNC(N)=N)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(N)=N)NC(=O)NC(Cc1ccccc1)C(O)=O)C(C)C
solubility	benzene: insoluble
	chloroform: insoluble
	DMSO: 20 mg/mL
	ethanol: sparingly soluble
	hexane: insoluble
	methanol: 20 mg/mL
	water: 20 mg/mL
storage temp.	2-8°C
technique(s)	cell culture \| mammalian: suitable

Application:	Concentrations for 50% inhibition (μg/ml): papain, 0.16 trypsin, 0.26 cathepsin A, 1.19 cathepsin B, 0.59 cathepsin D, 125 plasmin, >93 chymotrypsin and pepsin, >250 It also has been reported to inhibit calpain I, (porcine) with K_i = 1.4 μM
Biochem/physiol Actions:	Reversible inhibitor of serine/cysteine proteases and some trypsin-like serine proteases. Its action resembles leupeptin; however, its plasmin inhibition is less and its cathepsin A inhibition is more than that observed with leupeptin. Chronic administration of antipain can reduce the frequency of chemically induced transformation in BALB/c-/3T3 cells.
Formula variant:	Formula: C₂₇H₄₄N₁₀O₆ x 2 HCl
General description:	Antipain is a protease inhibitor isolated from actinomycetes. It inhibits thrombokinase and blood coagulation.
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Preparation Note:	Working concentration: 50 μg/ml Working solution: Soluble in water, methanol, or DMSO to 20 mg/ml. Sparingly soluble in ethanol, propanol, or butanol. Insoluble in benzene, chloroform (CHCl₃), hexane, or petroleum and ethyl ethers. Storage conditions (working solution): -15 to -25 °C Dilute solutions should be stored frozen in aliquots at -15 to -25 °C. Growth of microorganisms and repeated freezing should be avoided. Stable for approximately one month.
Preparation Note:	Solubility testing at 50 mg/ml in water yields a clear to slightly hazy yellow solution. It is reportedly soluble in methanol, water, and DMSO; less soluble in ethanol, butanol, and propanol; insoluble in benzene, hexane, and chloroform.⁸ A stock solution in water or buffer is stable for about a month at -20 °C. Dilute solutions should be stored on ice and kept for only a day because of the terminal aldehyde, which is subject to oxidation and racemization.
Reconstitution:	Soluble in water, methanol, or DMSO to 20 mg/ml. Sparingly soluble in ethanol, propanol, or butanol. Insoluble in benzene, chloroform (CHCl₃), hexane, or petroleum and ethyl ethers. Dilute solutions should be stored frozen in aliquots at -15 to -25 °C. Stable for approximately one month. CAUTION: DMSO (Dimethyl sulfoxide) will permeate the skin, carrying solubilized protease inhibitors. Always wear appropriate protection for eyes, skin, etc.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

mp	209-217 °C
Storage Temp.	2-8°C
UNSPSC	12352200

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