Antipain dihydrochloride
ROCHE/11004646001 - crystalline, suitable for cell culture, pkg of 10 mg
Synonym: Antipain dihydrochloride from microbial source; N-(Nα-Carbonyl-Arg-Val-Arg-al)-Phe
CAS Number: 37682-72-7
Empirical Formula (Hill Notation): C27H44N10O6 · 2 HCl
Molecular Weight: 677.62
MDL Number: MFCD00135957
Linear Formula: C27H44N10O6 · 2 HCl
Product Type: Chemical
form | crystalline |
InChI | 1S/C27H44N10O6.2ClH/c1-16 |
InChI key | YAHXZYICKJUJEO-BXLPLHKWSA |
manufacturer/tradename | Roche |
mol wt | Mr = 677.63 |
mp | 209-217 °C |
packaging | pkg of 10 mg |
Quality Level | 100 ![]() |
SMILES string | Cl[H].Cl[H].[H]C(=O)C(CCC |
solubility | benzene: insoluble |
chloroform: insoluble | |
DMSO: 20 mg/mL | |
ethanol: sparingly soluble | |
hexane: insoluble | |
methanol: 20 mg/mL | |
water: 20 mg/mL | |
storage temp. | 2-8°C |
technique(s) | cell culture | mammalian: suitable |
Application: | Concentrations for 50% inhibition (μg/ml): papain, 0.16 trypsin, 0.26 cathepsin A, 1.19 cathepsin B, 0.59 cathepsin D, 125 plasmin, >93 chymotrypsin and pepsin, >250 It also has been reported to inhibit calpain I, (porcine) with Ki = 1.4 μM |
Biochem/physiol Actions: | Reversible inhibitor of serine/cysteine proteases and some trypsin-like serine proteases. Its action resembles leupeptin; however, its plasmin inhibition is less and its cathepsin A inhibition is more than that observed with leupeptin. Chronic administration of antipain can reduce the frequency of chemically induced transformation in BALB/c-/3T3 cells. |
Formula variant: | Formula: C27H44N10O6 x 2 HCl |
General description: | Antipain is a protease inhibitor isolated from actinomycetes. It inhibits thrombokinase and blood coagulation. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Preparation Note: | Working concentration: 50 μg/ml Working solution: Soluble in water, methanol, or DMSO to 20 mg/ml. Sparingly soluble in ethanol, propanol, or butanol. Insoluble in benzene, chloroform (CHCl3), hexane, or petroleum and ethyl ethers. Storage conditions (working solution): -15 to -25 °C Dilute solutions should be stored frozen in aliquots at -15 to -25 °C. Growth of microorganisms and repeated freezing should be avoided. Stable for approximately one month. |
Preparation Note: | Solubility testing at 50 mg/ml in water yields a clear to slightly hazy yellow solution. It is reportedly soluble in methanol, water, and DMSO; less soluble in ethanol, butanol, and propanol; insoluble in benzene, hexane, and chloroform.8 A stock solution in water or buffer is stable for about a month at -20 °C. Dilute solutions should be stored on ice and kept for only a day because of the terminal aldehyde, which is subject to oxidation and racemization. |
Reconstitution: | Soluble in water, methanol, or DMSO to 20 mg/ml. Sparingly soluble in ethanol, propanol, or butanol. Insoluble in benzene, chloroform (CHCl3), hexane, or petroleum and ethyl ethers. Dilute solutions should be stored frozen in aliquots at -15 to -25 °C. Stable for approximately one month. CAUTION: DMSO (Dimethyl sulfoxide) will permeate the skin, carrying solubilized protease inhibitors. Always wear appropriate protection for eyes, skin, etc. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
mp | 209-217 °C |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |