Random Primed DNA Labeling Kit
ROCHE/11004760001
Synonym: nucleic acid labeling
Product Type: Chemical
| manufacturer/tradename | Roche |
| Quality Level | 100  |
| storage temp. | −20°C |
| usage | sufficient for 50 labeling reactions |
| Application: | Random Primed DNA Labeling Kit has been used to label probe or fragments shorter than 1 kb. |
| Application: | Random Primed DNA Labeling Kit is used to uniformly label plasmid or phage DNA with any [α-32P]-dNTP or modified dNTP. Labeled DNA probes with high specific activity are used in a variety of hybridization techniques including: • Screening of gene libraries • Southern, dot and northern blots • In situ hybridizations |
| General description: | Random primed DNA labeling kit is used for the radioactive and nonradioactive labeling of DNA with modified deoxyribonucleoside triphosphate using random oligonucleotides as primers. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 7 components. |
| Preparation Note: | Working solution: Preparation dNTP Stock Mix To avoid pipetting inaccuracy, due to low volumes, prepare a stock mix of unlabeled dNTPs. Aliquots should be stored at -15 to -25 °C. Avoid repeated freezing and thawing. • dATP, dGTP, dTTP mixture: For one labeling reaction pipette: 1 μl dATP 1 μl dGTP 1 μl dTTP to a reaction vial Preparation of DIG Stock Mix To avoid pipetting inaccuracy due to low volumes, prepare a DIG stock mix. To do this, mix digoxigenin-11-dUTP [3 mM] and dTTP (vial 5) 1:1. For each labeling reaction 1.6 μl are used. |
| Specificity: | Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or by heating to 65 °C for 10 minutes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41105500 |
| Components | Control DNA lambda, 20 μl 12.5 µg/ml; dATP, 50 μl 0.5 mM; dCTP, 50 μl 0.5 mM; dGTP, 50 μl 0.5 mM; dTTP, 50 μl 0.5 mM; Hexanucleotide mixture in 10x reaction buffer (100 μl); Klenow enzyme, labeling grade (100 U) |