Interferon-γ, human (hIFN-γ)
ROCHE/11040596001 - from human, 95% (SDS-PAGE), solution, >2 x 10^7 U/mg
Synonym: SYBR; qPCR
Product Type: Chemical
| assay | 95% (SDS-PAGE) |
| biological source | human |
| description | recombinant (E. coli) |
| form | solution |
| impurities | <0.1 EU/μgtested (LAL test) |
| manufacturer/tradename | Roche |
| mol wt | 16,700 Da |
| packaging | pkg of 100,000 U (5 μg, 1 ml) |
| potency | <0.05 ng/mL EC50 |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| specific activity | >2 x 10^7 U/mg |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | P01579  |
| Application: | Interferon-γ, human (hIFN-γ) has been used to stimulate mesenchymal stem cells (MSCs). It has also been used as a supplement Dulbecco′s modified Eagle′s medium/Ham′s medium for the cultivation of human colorectal carcinoma cell line T84 cells. |
| Application: | Interferon-γ, human (hIFN-γ), can be used to investigate IFN-γ activities in human cell systems. |
| Biochem/physiol Actions: | A broad range of biological activities has been attributed to IFN-γ (e.g., the establishment of the antiviral state, immunoregulatory functions, antiproliferative effects and inhibition of cell growth). The anti-proliferative effects of IFN-γ are superior to those of either IFN-α or IFN-β. Growth inhibition is dependent on cell type, dose, and length of exposure. One of IFN-γ’s primary functions might be as an immunoregulatory agent: IFN-γ induces MHC antigens on many cells, Fc-receptors on monocytes and macrophages, IL-2 receptors on T-cells, enhances activity of macrophages, polymorphonuclear leukocytes, T-lymphocytes, and NK-cells (MAF), and is also involved in the regulation of B-cells. |
| Biochem/physiol Actions: | Interferon-γ (IFN-γ) is an essential component of acquired immunity. A broad range of biological activities has been attributed to IFN-γ (e.g., the establishment of the antiviral state, immunoregulatory functions, antiproliferative effects and inhibition of cell growth). The anti-proliferative effects of IFN-γ are superior to those of either IFN-α or IFN-β. Growth inhibition is dependent on cell type, dose, and length of exposure. One of IFN-γ′s primary functions might be as an immunoregulatory agent: IFN-γ induces MHC antigens on many cells, Fc-receptors on monocytes and macrophages, IL-2 receptors on T-cells, enhances activity of macrophages, polymorphonuclear leukocytes, T-lymphocytes, and NK-cells (MAF), and is also involved in the regulation of B-cells. |
| General description: | IFN-γ is produced by T-lymphocytes stimulated by antigen or by T-cell mitogens. Under nondenaturating conditions its molecular weight values range from 32,000 to 73,000 indicating that recombinant human IFN-γ exists as a dimer or higher oligomers. |
| Other Notes: | EC 50 : <0.05ng/ml (hIFN-γ , NIH, reference standard, Gg 23–901-530) |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | 100,000U/ml or 1,000,000U/ml of Human IFN-γ in 0.1M phosphate buffer saline (PBS) (pH 7.0), 2.5% sucrose (w/v), and 2.5% human serum albumin (HSA) (w/v), filtered through a 0.2μm pore size membrane. |
| Preparation Note: | Working solution: Solvent is recommended in double-distilled water. Storage conditions (working solution): Stability in culture: Activity is present for at least 18 hours in culture medium. |
| Quality: | The raw material used for this preparation was tested for HBs antigen and for the presence of antibodies to HIV-1, HIV-2, and HCV found to be negative. Endotoxin level: <0.1EU/μg (LAL-test), <10EU/ml (LAL-test) Note: 1EU corresponds to 0.1ng |
| Sequence: | Chain Length 143 AA |
| Sequence: | The primary structure of recombinant human IFN-γ (143 amino acids) is identical to that of natural human IFN-γ (146 amino acids), however, recombinant IFN-γ has three amino acids less and is not glycosylated. Glycosylation is not essential for biological activity. |
| Specificity: | Human IFN-γ is effective on human cells, but not on mouse or rat cells. |
| Unit Definition: | EC50 definition: The amount of hIFN-γ that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN-γ reference standard (Gg 23– 901-530) (WISH cells-EMC virus/cytopathic effect) (1 unit equals ≤0.05 ng/ml). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Purity | 95% (SDS-PAGE) |
| activity | specific activity: >2 x 10^7 U/mg |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |