dGTP
ROCHE/11051466001 - =98%, solution, 100 mM, suitable for PCR, suitable for DNA sequencing
Synonym: 2′-Deoxyguanosine 5′-triphosphate trisodium salt solution; 2′-deoxy-guanosine-5′-triphosphate; deoxy-guanosine-5′-triphosphate, 2′-; dGTP-Na3
CAS Number: 93919-41-6
Empirical Formula (Hill Notation): C10H13N5Na3O13P3
Molecular Weight: 573.13
MDL Number: MFCD00080337
Linear Formula: C10H13N5Na3O13P3
Product Type: Chemical
| assay | =98% |
| concentration | 100 mM |
| description | Lithium salt |
| form | solution |
| InChI | 1S/C10H16N5O13P3.3Na/c11- |
| InChI key | IWGGLKOTEOCWQP-BIHLCPNHSA |
| manufacturer/tradename | Roche |
| packaging | pkg of 250 μL (25 μmol; 100 mM) |
| Quality Level | 100  |
| SMILES string | [Na+].[Na+].[Na+].NC1=Nc2 |
| storage temp. | −20°C |
| technique(s) | DNA amplification: suitable |
| DNA sequencing: suitable | |
| DNA synthesis: suitable | |
| PCR: suitable | |
| usage | sufficient for 6,250 standard reactions (PCR) |
| Application: | 2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques. |
| Application: | Use dGTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions. |
| General description: | 2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5’-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase. |
| General description: | dGTP is a 100 mM clear, colorless solution of the lithium salt (pH 7). dGTP solution in water (pH 7), 100 mM, lithium salt. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water. For your convenience, some of our deoxynucleotides are also available in: • nucleotide sets (all 4 nucleotides in separate vials) • ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides) They are also vital components of: • kits (dNTPs, buffer, and polymerase in separate vials) • ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)] |
| Quality: | Typical analysis: • ≥98% dGTP (HPLC), ≤1.5% dGDP (HPLC), ≤0.5% dGMP (HPLC) • Absorbance determination A250/A260, A280/A260, A290/A260 • DNase activity negative, according to the current Quality Control procedures • Suitability for PCR |
| Unit Definition: | 1 μmol dGTP = 13.7 A252 units |
| WGK Germany | nwg |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Purity | =98% |
| Storage Temp. | −20°C |
| UNSPSC | 41116100 |