DNA Molecular Weight Marker VI
ROCHE/11062590001 - pkg of 50 μg (in 200 μl), solution
Synonym: DNA marker
Product Type: Chemical
DNA Molecular Weight Marker VI (2% agarose gel)
| color | colorless |
| concentration | 250 μg/mL |
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 μg (in 200 μl) |
| Quality Level | 100  |
| solubility | water: miscible |
| storage temp. | −20°C |
| Application: | DNA Molecular Weight Marker VI is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by: • Restriction digests • PCR • RT-PCR |
| Biochem/physiol Actions: | Specificity: Product has 5′-phosphorylated ends (restriction fragments). Restriction site: Claevage sites and corresponding fragment lengths of pBR328: - Bgl I cut sites at: 935, 1169, 2399, 4575, 4809 => creating following fragment sizes, respectively: 234 bp, 1230 bp, 2176 bp, 234 bp, 1033 bp. - Hinf I cut sites at: 632, 852, 1006, 1304, 1757, 2274, 4040, 4434, 4732, 4886. => creating following fragment sizes, respectively: 220 bp, 154 bp, 298 bp, 453 bp, 517 bp, 1766 bp, 394 bp, 298 bp, 154 bp, 653 bp |
| General description: | DNA Molecular Weight Marker VI is a fragment mixture prepared by cleavage of pBR328 DNA with restriction endonucleases Bgl I and pBR328 DNA digested with Hinf I. It has a size range of 0.15 to 2.1kbp. The product is also available as DNA Molecular Weight Marker VI, Digoxigenin-labeled. |
| Other Notes: | 50 μg = 1A260 unit |
| Other Notes: | As determined by computer analysis of the pBR328 sequence, the mixture contains 15 DNA fragments with the following base pair lengths (1 base pair = 660 daltons) occuring once: 220, 394, 453, 517, 653, 1033, 1230, 1766, 2176 and fragments 154,234, and 298 occuring twice as a result of the digestion of pBR328 with both Bgl I and Hinf I. Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0). |
| Preparation Note: | Storage conditions (working solution): 2 to 8 °C Once thawed we recommend further storage at 2 to 8 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41105335 |