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Insulin-Transferrin-Sodium Selenite Supplement

ROCHE/11074547001 - suitable for cell culture, lyophilized, pkg of 50 mg (for 5 l medium)

Synonym: ITS

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-11074547001 50 mg
 $208.00
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assay >98% (Transferrin, SDS-PAGE)
  >99% (Insulin, HPLC)
  >99% (Sodium selenite)
biological source human serum (transferrin)
  synthetic (sodium selenite)
form lyophilized
manufacturer/tradename Roche
packaging pkg of 50 mg (for 5 l medium)
Quality Level 100 
recombinant expressed in human cells
solubility water: miscible
sterility non-sterile; 0.2 μm filtered
storage temp. 2-8°C
technique(s) cell culture | mammalian: suitable
UniProt accession no. P01317 
  P02786 
Application: The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added.
General description: The insulin-transferrin-sodium selenite (ITS) supplement comprises three of the most crucial growth factors for many cell types. Insulin is a component of serum-free media formulations for all primary cells and cell lines. It stimulates cell growth, increases fatty acid and glycogen synthesis in a serum-free medium. Transferrin is an essential growth factor for many cell types. Selenium is very often necessary for optimal cell growth. ITS permits an appreciable reduction in serum requirements for cellular growth. ITS is also an antioxidant that supports the growth of in vivo derived (IVD) embryos. Insulin promotes embryonic growth and metabolism. The iron transport in the embryo is mediated by transferrin. Sodium selenite protects from oxidative damage and inhibits lipid peroxidation. ITS is useful in human chondrocytes monolayer culture.
General description: The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio.
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Working concentration: Insulin, 5 μg/ml, transferrin, 5 μg/ml, sodium selenite, 5 ng/ml (3.0 x 10-8 M)

Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.
Preparation Note: The insulin-transferrin-sodium selenite supplement should be reconstituted in 5 ml or 25 ml sterile double-dist. water (1000 x concentrated stock solution).
Further dilution with culture medium.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) does not flash
Flash Point(C) does not flash
Purity >98% (Transferrin, SDS-PAGE); >99% (Insulin, HPLC); >99% (Insulin, HPLC); >99% (Sodium selenite)
Storage Temp. 2-8°C
UNSPSC 12352207

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