Neuraminidase (Sialidase)
ROCHE/11080725001 - from Vibrio cholerae
Synonym: Salidase
Product Type: Chemical
| application(s) | life science and biopharma sample preparation |
| biological source | Vibrio cholerae |
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | ~95 kDa |
| optimum pH | 5.5-6.2 |
| packaging | pkg of 1 U |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| suitability | suitable for ELISA applications |
| Application: | Neuraminidase has been used: • to remove cis-acting sialic acids in CHO (chinese hamster ovary) cells • for deglycosylation studies |
| Biochem/physiol Actions: | Hydrolyzes terminal N- or O-acyl-neuraminic acids that are α2,3-, α2,6-, or α2,8-linked to galactose, Hex, NAc, or N- or O-acylated neuraminyl residues in oligosaccharides/glycocon |
| General description: | Approximately 40 U/mg enzyme protein at 37 °C and pH 5.5, with N-acetyl-neuraminosyl-D-l |
| General description: | Neuraminidase is an acylneuraminyl hydrolase which hydrolyzes terminal N- or O-acylneuraminic acids which are α2,3-, α2,6-, or α2,8-linked (rate: α2,6 > α2,3 > α2,8) to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids. Noteworthy, for the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials (e.g., in cytology, on cell surfaces, viruses etc.). |
| Legal Information: | The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Other Notes: | One unit is the enzyme activity that hydrolyzes 1 μmol N-acetyl-neuraminosyl-D-l 10 mM N-acetyl-neuraminosyl-D-l |
| Physical form: | Solution in 50 mM sodium acetate, 154 mM sodium chloride, 9 mM calcium chloride, 0.1% Micr-O-Protect (w/v), human serum albumin, 25 mg/l, pH 5.5. The preparation contains 10 mM EDTA. Note: The serum used for this preparation was tested for HBs antigen and for the presence of antibodies to HIV-1, HIV-2, HCV, and found to be negative, according to the current quality control procedures. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.18 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |