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Phytohemagglutinin-M (PHA-M)

ROCHE/11082132001 - from Phaseolus vulgaris

Synonym: PHA-M

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11082132001 20 mg
$201.00
1/EA
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application(s) hematology
form lyophilized
manufacturer/tradename Roche
packaging pkg of 20 mg
Quality Level 100 
sterility non-sterile; 0.2 μm filtered
storage temp. 2-8°C
Application: Phytohemagglutinin (PHA) is a potent mitogen used to stimulate cell proliferation in lymphocyte cultures.
Application: Phytohemagglutinin-M (PHA-M) has been used in the activation of Jurkat and primary cells. It has also been used to treat polymorphonuclear blood cells(PMBCs)
Biochem/physiol Actions: Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes.
General description: Phytohemagglutinin (PHA), the lectin extract from the red kidney bean. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Working concentration: 2 - 10 μg/ml
Approximately 2 - 10 μg/ml for the stimulation of peripheral blood lymphocytes
Storage conditions (working solution): The reconstituted solution is stable for 2 weeks at 2 to 8 °C or for several months when stored in aliquots at -15 to -25 °C.
Reconstitution: PHA-M should be reconstituted in sterile double-dist. water (final concentration: 2–10 mg/ml). Further dilution with medium or PBS (phosphate buffered saline).
Specifications: Biological activity: <10 μg/ml for maximal stimulation of DNA synthesis (BrdU incorporation) with human peripheral blood lymphocytes.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) does not flash
Flash Point(C) does not flash
Storage Temp. 2-8°C
UNSPSC 23201100

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