BM Condimed H1
ROCHE/11088947001 - Hybridoma Cloning Supplement
Synonym: hybridoma cloning supplement
Product Type: Chemical
BM Condimed H1
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 100 mL (10x) |
| Quality Level | 100  |
| solubility | water: miscible |
| sterility | 0.2 μm filtered |
| storage temp. | −20°C |
| technique(s) | cell culture | hybridoma: suitable |
| Analysis Note: | Biological activity: Assayed for its ability to promote the growth of freshly fused hybridoma cells. |
| Application: | BM Condimed H1 is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. BM Condimed H1 replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen. |
| Biochem/physiol Actions: | BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning. |
| Features and Benefits: | Contents Solution in RPMI 1640, filtered through 0.2μm pore size membrane |
| General description: | BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution in RPMI 1640, filtered through 0.2 μm pore size membrane |
| Preparation Note: | Working concentration: 10% (v/v) in culture medium that contains 10 - 20% FBS. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |