Streptavidin-AP conjugate
ROCHE/11089161001 - solution, pkg of 1 mL (1,000U)
Synonym: streptavidin
Product Type: Chemical
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 1 mL (1,000U) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | Streptavidin-AP has been used to detect biotinylated compounds using: • ELISA • Immunohistocytochemistr • Western blot |
| General description: | Streptavidin is covalently coupled to alkaline phosphatase (AP) for the preparation of streptavidin-AP-conjugate Alkaline Phosphatase Alkaline Phosphatase is often used as a label in enzyme immunoassays. AP converts the sensitive substrate X-phosphate/NBT into a stable, water soluble product. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution, stablilized in 50 mM Triethanolamine buffer, 3 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 10 mg/ml bovine serum albumin, pH 7.6. |
| Preparation Note: | Working solution: 50 - 1,000 mU/ml (i.e., stock solution can be diluted). Dilute the conjugate stock solution in 100 mM Tris-HCl, 150 mM NaCl, pH 7.5. Working concentration: Working concentration of conjugate will depend on the application and substrate. Indicated concentrations should be taken as a guideline. ELISA: 50 - 200 mU/ml (1:5000 - 1:20,000 dilution) Immunohisto/cytochemistry Western blot: 200 - 500 mU/ml (1:2000 - 1:5000 dilution) Stability: The concentrated stock solution is stable when stored at 2 to 8°C until the expiration date printed on the label. Do not freeze! |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |