Biotin-16-dUTP
ROCHE/11093070910
Synonym: Bio-16-dUTP tetralithium salt; 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′-deoxyuridine 5′-triphosphate
CAS Number: 136632-31-0
Empirical Formula (Hill Notation): C32H52N7O18P3S
Molecular Weight: 947.78
Linear Formula: C32H52N7O18P3S
Product Type: Chemical
Biotin-16-dUTP
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 μL (50 nmol; 1mM) |
| Quality Level | 100  |
| storage temp. | −20°C |
| Analysis Note: | Absorption: The spectral data of Biotin-16-dUTP (at pH 7.3): Emax241: E = 10.700; Emax292: E = 7.000; Emax265: E = 4.400; A292/265 : 1.6. |
| Application: | For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation. • Biotin-16-dUTP is used as asubstrate for: Terminal Transferase • DNA polymerase I (holoenzyme and Klenow fragment) • Taq DNA polymerase • Reverse transcriptase (e.g., Transcriptor) Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR. The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling. • Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate • Biotin Luminescence Detection Kit |
| Biochem/physiol Actions: | Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency. |
| General description: | Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Quality: | Typical analysis: At least 85% deoxynucleoside triphosphate (Biotin-16-dUTP) (HPLC, area%). Notes: For your convenience, biotin-dUTP is also available in convenient labeling mixes (single solution with conventional dNTPs and biotin-16-dUTP), and technique-specific mixes (nucleotides, buffers, and enzymes in a single vial). |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41116100 |