Anti-Digoxigenin-AP, Fab fragments
ROCHE/11093274910 - from sheep
Synonym: anti-digoxigenin; digoxigenin
Product Type: Chemical
Anti-Digoxigenin-AP, Fab fragments
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | sheep |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | solution |
| isotype | IgG |
| manufacturer/tradename | Roche |
| packaging | pkg of 200 μL (150 U) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Analysis Note: | • Cross reactivity to digitoxin and digitoxigenin: <1 % • No cross reactivity with other human estrogen or androgen steroids, e.g. estradiol or testosterone • Cross reactivity with digoxin: not known • Conjugate does not bind to itself at all • Normally one molecule of the conjugate binds to one molecule digoxigenin, although ther are two possible binding sites for digoxigenin • Nonspecific binding to RNA is not expected |
| Application: | Use Anti-Digoxigenin-AP, Fab fragments for the detection of digoxigenin-labeled compounds using: • cDNA array • Colony/plaque hybridization • Dot blot • ELISA • Gel shift assay • Immunohistocytochemistr • In situ hybridization • Nonradioactive DNA sequencing blot • Northern blot • RNase protection assay • Southern blot • Western blot • Fluorescent in situ hybridization • Section in situ hybridization and whole mount in situ hybridization • Electrophoretic mobility shift assay |
| General description: | Digoxigenin is a hapten, useful in labeling and detection of nucleic acids. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to alkaline phosphatase. Anti-Digoxigenin-AP, Fab fragments are useful for the detection of digoxigenin-labeled compounds. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer. Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline: • Dot blot: 150 mU/ml • ELISA: 150 to 300 mU/ml • Immunohistocytochemistr • In situ hybridization: 1.5 to 7.5 U/ml • Southern blot: 150 mU/ml • Western blot: 250 to 500 mU/ml Working solution: Northern blot, Southern blot 100 mM Maleic acid, 150 mM NaCl, pH 7.5. Western blot 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 other applications 100 mM Tris-HCl, 150 mM NaCl, pH 7.5 1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Storage conditions (working solution): Diluted conjugate is stable at 2 to 8 °C for 12 hours. Always prepare freshly! |
| Specificity: | The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens. Heat inactivation: yes |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |