DIG DNA Labeling and Detection Kit
ROCHE/11093657910
Synonym: dna labeling and detection kit, dig
Product Type: Chemical
Detection of a single-copy gene (Β-actin) in total human DNA using the standard protocol.
Sensitivity of DIG detection.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category |  , Aligned |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| manufacturer/tradename | Roche |
| Quality Level | 100 |
| storage temp. | −20°C |
| sustainability | Greener Alternative Product |
| usage | sufficient for 25 labeling reactions |
| sufficient for 50 blots |
| Application: | DIG DNA Labeling and Detection Kit has been used in a variety of hybridization techniques: • Southern blots • Northern blots • Dot blots • Colony and plaque screening DIG-labeled hybrids are detected with an anti-DIG-alkaline phosphatase conjugate and the substrates NBT (nitroblue tetrazolium salt) and BCIP (5-bromo-4-chloro-3-indol |
| Biochem/physiol Actions: | Sensitivity and specificity: The standard labeling reaction with 1 μg template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at +37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction. |
| General description: | DIG DNA Labeling and Detection Kit is a convenient kit for random-primed labeling of DNA with digoxigenin-deoxyuridine triphosphate (dUTP), alkali-labile and color detection of hybrids by enzyme immunoassay. In this method, the complementary strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of random oligonucleotides as primers. Contents: • Unlabeled Control DNA 1, 100 μg/ml • Unlabeled Control DNA 2, 200 μg/ml • DNA Dilution Buffer • DIG-labeled Control DNA, 5.2 μg/ml • 10x Hexanucleotide Mix • 10x dNTP Labeling Mixture • Klenow Enzyme, Labeling grade, 2 U/μl • Anti-digoxigenin-AP-conjugate, 750 U/ml • NBT/BCIP Concentrated Stock Solution • Blocking Reagent |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 10 components. |
| Preparation Note: | Working concentration: Dilution of Antibody 1:5,000 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41105500 |
| Components | Unlabeled Control DNA 1 100 µg/ml; Unlabeled Control DNA 2 200 µg/ml; DNA Dilution Buffer; DIG-labeled Control DNA 5.2 µg/ml; Hexanucleotide Mix 10x concentrated; dNTP Labeling Mixture 10x concentrated; Klenow Enzyme, Labeling grade 2 U/µl; Anti-digoxigenin-AP-conjugate antibody 750 U/ml; NBT/BCIP Concentrated Stock Solution; Blocking Reagent |