DIG DNA Labeling and Detection Kit
ROCHE/11093657910
Synonym: dna labeling and detection kit, dig
Product Type: Chemical
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greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry ![]() |
manufacturer/tradename | Roche |
Quality Level | 100 ![]() |
storage temp. | −20°C |
usage | sufficient for 25 labeling reactions |
sufficient for 50 blots |
Application: | DIG DNA Labeling and Detection Kit has been used in a variety of hybridization techniques: • Southern blots • Northern blots • Dot blots • Colony and plaque screening DIG-labeled hybrids are detected with an anti-DIG-alkaline phosphatase conjugate and the substrates NBT (nitroblue tetrazolium salt) and BCIP (5-bromo-4-chloro-3-indol |
General description: | DIG DNA Labeling and Detection Kit is a convenient kit for random-primed labeling of DNA with digoxigenin-deoxyuridine triphosphate (dUTP), alkali-labile and color detection of hybrids by enzyme immunoassay. In this method, the complementary strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of random oligonucleotides as primers. Contents: • Unlabeled Control DNA 1, 100 μg/ml • Unlabeled Control DNA 2, 200 μg/ml • DNA Dilution Buffer • DIG-labeled Control DNA, 5.2 μg/ml • 10x Hexanucleotide Mix • 10x dNTP Labeling Mixture • Klenow Enzyme, Labeling grade, 2 U/μl • Anti-digoxigenin-AP-conjugate, 750 U/ml • NBT/BCIP Concentrated Stock Solution • Blocking Reagent |
General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Packaging: | 1 kit containing 10 components. |
Preparation Note: | Working concentration: Dilution of Antibody 1:5,000 |
Specificity: | Sensitivity and specificity: The standard labeling reaction with 1 μg template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at +37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 41105500 |
Components | Unlabeled Control DNA 1 100 µg/ml; Unlabeled Control DNA 2 200 µg/ml; DNA Dilution Buffer; DIG-labeled Control DNA 5.2 µg/ml; Hexanucleotide Mix 10x concentrated; dNTP Labeling Mixture 10x concentrated; Klenow Enzyme, Labeling grade 2 U/µl; Anti-digoxigenin-AP-conjugate antibody 750 U/ml; NBT/BCIP Concentrated Stock Solution; Blocking Reagent |