Blocking Reagent
ROCHE/11096176001 - For nucleic acid hybridization and detection
Synonym: blocking reagent
Product Type: Chemical
Blocking Reagent
| form | powder |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 g |
| Quality Level | 100  |
| storage temp. | 20-25°C |
| Application: | The Blocking Reagent is used to decrease the background in nonradioactive filter hybridization and the detection of nucleic acid hybrids. It has also been used as a blocking agent in immunohistochemistry. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Dilution of Antibody 1:5,000 Working solution: To prepare a 10x blocking stock solution Blocking Reagent is dissolved in maleic acid buffer to a final concentration of 10% (w/v) with shaking and heating either on a heating block or in a microwave oven. Storage conditions (working solution): 10x stock solution of Blocking Reagent is autoclaved and stored at 2 to 8 °C. Alternativly, aliquots can be stored at -15 to -25 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 20-25°C |
| UNSPSC | 41116100 |