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ABTS™ Buffer

ROCHE/11112597001

Synonym: ABTS

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-11112597001 16.7 g
 $0.00
1/EA
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form powder
manufacturer/tradename Roche
packaging pkg of 16.7 g
Quality Level 100 
storage temp. 2-8°C
Application: ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme. Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.
General description: 2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA, using ABTS, is a highly sensitive, specific and reproducible technique.
Legal Information: ABTS is a trademark of Roche
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Working solution: Dissolve the contents of one bottle substrate for ABTS in 100 ml double-distilled water. This concentrate is stable when stored in aliquots at -15 to -25 °C.
To obtain the working solution, dilute one aliquot in a ratio of 1:10 with double-dist. water. Dissolve one ABTS tablet (50 mg) in 50 ml of the working solution. This solution has a light-green color. In microtiter plates the solution is colorless. A405 nm/1 cm should be < 0.16.
Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
Storage Temp. 2-8°C
UNSPSC 12352204

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