RNase, DNase-free

ROCHE/11119915001 - from bovine pancreas

Synonym: Rnase

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-11119915001	500 µg	$148.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

biological source	bovine pancreas
form	solution
manufacturer/tradename	Roche
packaging	pkg of 500 μg (1 ml)
Quality Level	100
specific activity	≥30 units/mg protein
storage temp.	−20°C
technique(s)	DNA purification: suitable

Application:	RNase, DNase-free, efficiently removes contaminating RNA from plasmid or genomic DNA preparations.
General description:	Pyrimidine-specific endoribonuclease that acts on single-stranded RNA. RNase, DNase-free, is a heterogeneous mixture of ribonucleases that has been prepared free of deoxyribonuclease activity according to the current Quality Control procedures. RNase, DNase-free, is particularly well suited for use in DNA isolation procedures. Before use, most RNase preparations must be boiled to remove DNase activity. This preparation of RNase does not need to be boiled; it can be used directly from the vial.
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Physical form:	Solution, 500 μg/ml, in 10 mM Tris-HCl, 5 mM CaCl₂, 50% glycerol (pH 7.0).
Preparation Note:	Working concentration: The optimal working concentration for RNase, DNase free, is 2 to 5 μg/ml. The reaction volume will vary for different applications. Some suggested guidelines are given below: For small-scale isolation of plasmid DNA ("miniprep" from a 1.5 ml bacterial culture), use 0.5 μl of RNase, DNase-free in a reaction volume of 50 μl. • To isolate plasmid DNA from a 100 ml bacterial culture, use 8 μl of RNase, DNase-free in a reaction volume of 2 ml. • To isolate genomic DNA from cultured mammalian cells (5 x 10⁷ cells), use 8 μl of RNase, DNase-free in a reaction volume of 2 ml. Working solution: Storage and Dilution Buffer: 10 mM Tris-HCl, 5 mM CaCl₂, 50% glycerol (v/v), pH 7.0.
Unit Definition:	One Kunitz unit is the amount of enzyme that causes a decrease in absorbance of A₀ to A₁ within one minute under the assay conditions. A₀ to A₁ corresponds to the total conversion, A₁ being the final absorbance. One unit produces a decrease in absorbance at 260 nm, which is equivalent to a total conversion of RNA to oligonucleotides in one minute at +25 °C.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	No data available
Flash Point(C)	No data available

activity	specific activity: ≥30 units/mg protein
Storage Temp.	−20°C
UNSPSC	41105600

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