ATP-γ-S
ROCHE/11162306001 - =90% (HPLC), solution, pkg of 200 μL (20 μmol)
Synonym: adenosine-5′-o-(3-thio-triphosphate)
Product Type: Chemical
| λmax | 260 nm at 100 mmol/L |
| assay | 90% (HPLC) |
| description | C10H16N5O12P3SLi (Formula) |
| Lithium salt | |
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | 523.2 |
| packaging | pkg of 200 μL (20 μmol) |
| Quality Level | 100  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Analysis Note: | Contaminants: ≤10% ADP (HPLC) |
| Application: | ATP-γ-S has been used:• to treat THP-1 macrophage cell lines for testing if endoplasmic reticulum (ER) stress causes the production of interleukin (IL)-1β • in amide hydrogen exchange (HX) experiments coupled to mass spectrometry and study the cyclomarin A (CymA)-induced structural changes in NMtb-ClpC • as a supplement in CMG-binding buffer for gel-based DNA unwinding assays |
| Biochem/physiol Actions: | Adenosine 5′-O-(3-thiotriphosphate) (ATP-γ-S) is a substrate and inhibitor of ATP-dependent enzyme systems. It is hydrolyzed very slowly by phosphatases and most ATPases. Once thiophosphorylated, proteins are resistant to protein phosphatases. ATP-γ-S serves as a damage-associated molecular pattern molecule (DAMP) and NLR family pyrin domain containing 3 (NLRP3) inflammasome activator. ATP-γ-S may serve as a substrate for RNA-stimulated nucleotide hydrolysis and RNA unwinding activities of eukaryotic initiation factor-4A (eIF4A). |
| General description: | Adenosine 5′-O-(3-thiotriphosphate) |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Purity | 90% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |