DOTAP Liposomal Transfection Reagent
ROCHE/11202375001 - >99% (TLC), liquid, suitable for transfection
Synonym: DOTAP methosulfate; liposomal transfection reagent, dotap; transfection reagent, dotap; N-
CAS Number: 144189-73-1
Empirical Formula (Hill Notation): C43H83NO8S
Molecular Weight: 774.19
MDL Number: MFCD16660441
Linear Formula: C43H83NO8S
Product Type: Chemical
DOTAP Liposomal Transfection Reagent
Influence of transfection time on CAT expression. 1 x 106 HeLa cells in 6-cm Petri dishes were transfected with 5 μg pSV2-CAT, and 30 μl DOTAP Liposomal Transfection Reagent (1 mg/mL) in 6 mL of culture medium that contains 10% FCS. The cells were incubated for different periods of time (A-G: 0-6 hours; H: 6-hour culture medium without FCS), then the medium aspirated, new culture medium was added, and the cells incubated for an additional 40 hours. Afterwards, the cells were lysed and CAT expression was analyzed using the CAT ELISA.
| assay | >99% (TLC) |
| description | N-[1-(2,3-Dioleoyloxy)pro |
| form | liquid |
| InChI | 1S/C42H80NO4.CH4O4S/c1-6- |
| InChI key | RSMRWWHFJMENJH-LQDDAWAPSA |
| manufacturer/tradename | Roche |
| packaging | pkg of 5 × 400 μL (10 U/μl) |
| Quality Level | 100  |
| SMILES string | [H]C(C[N+](C)(C)C)(OC(CCC |
| storage temp. | 2-8°C |
| technique(s) | transfection: suitable |
| Application: | Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded. |
| Application: | The DOTAP (N-[1-(2,3-Dioleoyloxy)pr |
| Features and Benefits: | • Easy to use: The lipid dispersion is simply mixed with the DNA solution, then applied directly to the cells • Highly effective: 5 to 100-fold more effective than the calcium phosphate or DEAE-dextran method • Gentle: No cytotoxic effects • Flexible: Equally effective in the presence or absence of serum. Effective on a wide range of species (e.g., insect cells, mammalian cells) and a variety of different cell types. Can be used for either transient or stable transfection procedures. • Aqueous dispersion (liposomes) in MBS (MES-buffered saline, pH 6.2) (bottled under argon), 1mg/ml, sterile-filtered. |
| General description: | DOTAP Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. Unlike other liposomal reagents, it produces highly efficient transfections both in the presence and absence of serum. It can also be successfully used for in vivo applications. Mixing DOTAP with DNA results in spontaneously formed stable complexes. These complexes can be added directly to the cell culture medium. This method of DNA transfer is very gentle, avoiding the cytotoxic effects commonly encountered with lipofection or other transfection methods. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Approximately 5 - 10μg DOTAP/μg DNA, and 10 - 20μg DOTAP/ml cell culture medium. The optimal working concentration is dependent on several parameters, including the cell line being used, concentration and type of nucleic acid (DNA, RNA), and incubation time. It is important to optimize the transfection conditions for the individual cell type studied. Cytotoxicity: Not cytotoxic up to a concentration of 100μg/ml (PBLs, HeLa cells). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | >99% (TLC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106502 |