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ABTS™ Buffer

ROCHE/11204530001 - solution, pkg of 125 mL

Synonym: buffer

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-11204530001 125 mL
 $64.30
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form solution
manufacturer/tradename Roche
packaging pkg of 125 mL
Quality Level 100 
shipped in wet ice
storage temp. 2-8°C
Application: ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme. Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.
General description: 2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS acts as a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS is a highly sensitive, specific and reproducible technique.
Legal Information: ABTS is a trademark of Roche
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Working concentration: Flow cytometry: 0.5 μg/100 μl (106 cells); Immunohistocytochemistry: 50 μg/ml
Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of ABTS buffer. This solution has a light-green color. In microtiter plates, the solution is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
RIDADR NONH for all modes of transport
WGK Germany nwg
Flash Point(F) No data available
Flash Point(C) No data available
Storage Temp. 2-8°C
UNSPSC 12352204

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