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DNA Molecular Weight Marker VII

ROCHE/11209264001 - pkg of 50 μg (in 200 μl), solution

Synonym: DNA marker

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11209264001 50 µg
$186.00
1/EA
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DNA Molecular Weight Marker VII (1% agarose gel)

 

color colorless
concentration 250 μg/mL
form solution
manufacturer/tradename Roche
packaging pkg of 50 μg (in 200 μl)
Quality Level 100 
solubility water: miscible
storage temp. −20°C
Application: DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
• Restriction digests
• PCR
• RT-PCR
General description: Contents
Ready-to-use solution, 250 μg/ml, in TE buffer (Tris-HCl, 1 mM EDTA, pH 8.0).
General description: Fragment mixture prepared by cleavage of SPP1-DNA with restriction endonuclease EcoR I.
The product is also available as DNA Molecular Weight Marker VII, Digoxigenin-labeled.
Size Range: 0.081 to 8.57 kbp
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Storage conditions (working solution): 2 to 8 °C
Once thawed store at 2 to 8 °C.
Sequence: The mixture contains seventeen fragments with the following base pair lengths (1 base pair = 660 daltons): 81, 359, 492, 710, 718*, 992, 1164, 1482, 1515, 1882, 1953, 2799, 3639, 4899, 6106, 7427, and 8576 as determined by computer analysis of the SSP1 sequence.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
*Alonso, J.C. and Trautner, T.A., personal communication of unpublished results, the 718-bp fragment is present in non-stoichometric amounts.
Unit Definition: 50 μg = 1A260 unit
RIDADR NONH for all modes of transport
WGK Germany nwg
Flash Point(F) does not flash
Flash Point(C) does not flash
Storage Temp. −20°C
UNSPSC 41105335

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