DNA Molecular Weight Marker VII
ROCHE/11209264001 - pkg of 50 μg (in 200 μl), solution
Synonym: DNA marker
Product Type: Chemical
color | colorless |
concentration | 250 μg/mL |
form | solution |
manufacturer/tradename | Roche |
packaging | pkg of 50 μg (in 200 μl) |
Quality Level | 100 |
solubility | water: miscible |
storage temp. | −20°C |
Application: | DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by: • Restriction digests • PCR • RT-PCR |
General description: | Contents Ready-to-use solution, 250 μg/ml, in TE buffer (Tris-HCl, 1 mM EDTA, pH 8.0). |
General description: | Fragment mixture prepared by cleavage of SPP1-DNA with restriction endonuclease EcoR I. The product is also available as DNA Molecular Weight Marker VII, Digoxigenin-labeled. Size Range: 0.081 to 8.57 kbp |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Preparation Note: | Storage conditions (working solution): 2 to 8 °C Once thawed store at 2 to 8 °C. |
Sequence: | The mixture contains seventeen fragments with the following base pair lengths (1 base pair = 660 daltons): 81, 359, 492, 710, 718*, 992, 1164, 1482, 1515, 1882, 1953, 2799, 3639, 4899, 6106, 7427, and 8576 as determined by computer analysis of the SSP1 sequence. Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. *Alonso, J.C. and Trautner, T.A., personal communication of unpublished results, the 718-bp fragment is present in non-stoichometric amounts. |
Unit Definition: | 50 μg = 1A260 unit |
RIDADR | NONH for all modes of transport |
WGK Germany | nwg |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
Storage Temp. | −20°C |
UNSPSC | 41105335 |