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Laminin

ROCHE/11243217001 - from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

Synonym: Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

CAS Number: 114956-81-9
MDL Number: MFCD00081739
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11243217001 1 mg
$385.00
1/EA
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assay ≥90% (SDS-PAGE)
biological source mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)
form solution
manufacturer/tradename Roche
mol wt 900 kDa
packaging pkg of 1 mg (2 ml)
Quality Level 100 
sterility sterile; sterile-filtered
storage temp. −20°C
technique(s) cell culture | mammalian: suitable
UniProt accession no. Q61292 
Application: Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Application: Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
General description: Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.

Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.
Other Notes: 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research 
Physical form: Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile
Physical form: Solution, filtered through 0.2 ?m pore size membrane
Preparation Note: Working concentration: 2 to 5 μg/cm2
2 to 5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.
Quality: Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)
Sequence: Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.
RIDADR NONH for all modes of transport
WGK Germany nwg
Flash Point(F) does not flash
Flash Point(C) does not flash
Purity ≥90% (SDS-PAGE)
Storage Temp. −20°C
UNSPSC 12352207

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