Laminin
ROCHE/11243217001 - from mouse Engelbreth-Holm-Swarm (EHS) sarcoma
Synonym: Laminin from Engelbreth-
CAS Number: 114956-81-9
MDL Number: MFCD00081739
Product Type: Chemical
assay | ≥90% (SDS-PAGE) |
biological source | mouse (Engelbreth-Holm-Swarm (EHS) sarcoma) |
form | solution |
manufacturer/tradename | Roche |
mol wt | 900 kDa |
packaging | pkg of 1 mg (2 ml) |
Quality Level | 100 ![]() |
sterility | sterile; sterile-filtered |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
UniProt accession no. | Q61292 ![]() ![]() |
Application: | Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives. |
Application: | Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives. |
General description: | Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. Biological activity: Tested for the promotion of adherence of HT-1080 cells. Species specificity: Active on most mammalian cells. |
Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research ![]() ![]() |
Physical form: | Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile |
Physical form: | Solution, filtered through 0.2 ?m pore size membrane |
Preparation Note: | Working concentration: 2 to 5 μg/cm2 2 to 5 μg/cm2 for the coating of cell culture vessels Storage conditions (working solution): 2 to 8 °C The undiluted solution is stable at 2 to 8 °C for at least 3 months. The solution should be thawed carefully at 2 to 8 °C or on ice. |
Quality: | Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1) |
Sequence: | Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated. |
RIDADR | NONH for all modes of transport |
WGK Germany | nwg |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
Purity | ≥90% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352207 |