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Phytohemagglutinin-L (PHA-L)

ROCHE/11249738001 - from Phaseolus vulgaris

Synonym: PHA-L

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-11249738001 5 mg
 $421.00
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application(s) hematology
assay >95% (SDS-PAGE)
form lyophilized (clear, colorless solution after reconstitution)
manufacturer/tradename Roche
packaging pkg of 5 mg
Quality Level 100 
sterility non-sterile; 0.2 μm filtered
storage temp. 2-8°C
Application: Phytohemagglutinin-L (PHA-L) consists of only L-type subunits (isolectin L4, "leuko-agglutinin"), and is well suited for high-efficiency induction and the functional analysis of T-lymphocytes. It is highly purified and tested for high-efficiency stimulation of human lymphocytes. It is also used as positive controls for peripheral blood mononuclear cells (PBMC) stimulation.
Biochem/physiol Actions: Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes.
Features and Benefits: Biological activity: <5μg/ml for maximal stimulation of DNA synthesis (BrdU incorporation) with human peripheral blood lymphocytes
General description: PHA-L is purified from Phaseolus vulgaris (red kidney bean) by standard chromatographic techniques.
Phytohemagglutinin (PHA), the lectin extract from the red kidney bean, consists of only L-type subunits (isolectin L4, "leuko-agglutinin"). It is an N-acetylgalactosamine/galactose sugar-specific lectin. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Other Notes: PHA-L is a tetramer of 4 L-type subunits held together by noncovalent forces.
Preparation Note: Working concentration: 1 to 5 μg/ml
Approximately 1 to 5 μg/ml for the stimulation of human peripheral blood lymphocytes
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
RIDADR NONH for all modes of transport
WGK Germany WGK 2
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity >95% (SDS-PAGE)
Storage Temp. 2-8°C
UNSPSC 23201100

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