Advanced Search



Hexanucleotide Mix

ROCHE/11277081001 - solution, pkg of 100 μL, sufficient for 50 labeling reactions

Synonym: nucleotide mix

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11277081001 100 µL
$83.20
1/EA
Add To Favorites

 

color colorless
form solution
manufacturer/tradename Roche
packaging pkg of 100 μL
Quality Level 100 
solubility water: miscible
storage temp. −20°C
technique(s) cDNA synthesis: suitable (first strand)
  hybridization: suitable
  Northern blotting: suitable
  Southern blotting: suitable
usage sufficient for 50 labeling reactions
Analysis Note: Absorption: 62.5 A260 units correspond to 2.5 mg/ml of hexanucleotides.
Application: Hexanucleotide Mix is a mixture of hexanucleotides of all possible sequences for random-primed DNA labeling.
Labeled DNA probes with high specific activity are used in a variety of hybridization techniques:
• Screening of gene libraries
• Southern and northern blots
• In situ hybridizations
• RT-PCR
• Generation of cDNA libraries
• Synthesis of first-strand cDNA
• in the determination of vector titer
• Second strand synthesis
Features and Benefits: The product is a 10x concentrated mixture of random hexanucleotides. Statistically, the mix may contain up to 4,096 different hexanucleotides, but these are probably present in differing amounts.

Contents
10x concentrated mixture of hexanucleotides (62.5 A260 units/ml) in reaction buffer [0.5M Tris- HCl, 0.1M MgCl2, 1mM dithioerythritol (DTE), 2mg/ml BSA, pH 7.2 (+20°C)]
Note: The mix is identical to that supplied in vial 5 of the DIG DNA Labeling and Detection Kit and of the DIG DNA Labeling Kit and in vial 6 of the Random Primed DNA Labeling Kit.
General description: Convenient oligonucleotide mixture for rapid random-primed labeling of DNA with radioactive, digoxigenin- or biotin-labeled nucleotides. In this method, the complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Assay Time
Standard labeling (radioactive): 50 minutes
Labeling assay with digoxigenin-11-dUTP: 80 minutes

Sample Materials
• DNA fragments
• Linearized plasmid DNA
• λDNA
Note: The length of the DNA fragments to be labeled does not influence the reaction. DNA fragments of 100bp length are labeled equally well as linearized plasmid- or λ-DNA. The input DNA serves solely as template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10ng) using this method.

Synthesis: All 4 bases are used to synthesize this random hexanucleotide mix. In the initial reaction, starter nucleotides are linked to a solid phase support. In subsequent coupling reactions, equimolar amounts of the 4 dNTPs are linked to the starter nucleotides until hexamers are generated. The hexamers are then released from the solid phase support.
Post-synthesis: The oligonucleotides are HPLC purified, desalted, and 5′-phosphorylated.
Principle: The "random primed" DNA labeling method developed by Feinberg and Vogelstein is based on the hybridization of a mixture of all possible hexanucleotides to the DNA to be labeled. All sequence combinations are represented in the hexanucleotide primer mixture, which leads to binding of primer to the template DNA in a statistical manner. Thus, an equal degree of labeling along the entire length of the template DNA is guaranteed. The complementary strand is synthesized from the 3′-OH termini of the random hexanucleotide primer using Klenow enzyme, labeling grade. Modified deoxyribonucleoside triphosphates ([32P], [35S],[3H], or [125I] digoxigenin- or biotin-labeled) present in the reaction are incorporated into the newly synthesized complementary DNA strand.
Quality: Function tested in the Random Primed DNA Labeling Kit.
Specificity: Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or by heating to 65 °C for 10 minutes.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) No data available
Flash Point(C) No data available
Storage Temp. −20°C
UNSPSC 41106300

The following items have been added to your cart:

Choose a favorite list for this item:

Catalog Number Description Price
$

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.


Quick Order

* Required


New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.

800-334-7725
office@krackeler.com


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top