DNase I
ROCHE/11284932001 - from bovine pancreas
Synonym: Dnase
Product Type: Chemical
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| biological source | bovine pancreas |
| form | lyophilized |
| manufacturer/tradename | Roche |
| packaging | pkg of 100 mg |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | DNase I has been used for tissue dissociation in conjunction with collagenases. |
| Biochem/physiol Actions: | During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping. |
| Features and Benefits: | • Lyophilized • Filtered through 0.2 μm pore size membrane before lyophilization • Glycoprotein and double-strand-specific endonuclease |
| General description: | DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: Bivalent metal ions (Ca2+, Mg2+) Working concentration: 0.01 to 1 mg/ml Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+. |
| Reconstitution: | Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium. |
| Unit Definition: | One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.21.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |