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DNase I

ROCHE/11284932001 - from bovine pancreas

Synonym: Dnase

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11284932001 100 mg
$173.00
1/EA
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biological source bovine pancreas
form lyophilized
manufacturer/tradename Roche
packaging pkg of 100 mg
Quality Level 100 
storage temp. 2-8°C
technique(s) cell culture | mammalian: suitable
Application: DNase I has been used for tissue dissociation in conjunction with collagenases.
Biochem/physiol Actions: During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.
Features and Benefits: • Lyophilized
• Filtered through 0.2 μm pore size membrane before lyophilization
• Glycoprotein and double-strand-specific endonuclease
General description: DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Activator: Bivalent metal ions (Ca2+, Mg2+)
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.
Reconstitution: Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.
Unit Definition: One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions.
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 3.1.21.1   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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