Fluorescein-12-dUTP
ROCHE/11373242910 - ≥85% (HPLC), suitable for ELISA, suitable for hybridization, solution
Synonym: Fluorescein-12-dUTP; dUTP
Product Type: Chemical
| assay | ≥85% (HPLC) |
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | Mr 1018.4 (fluorescein-12-dUTP-Li4) |
| packaging | pkg of 25 μL (25 nmol; 1mM) |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| hybridization: suitable |
| Analysis Note: | Typical analysis: At least 85% Fluorescein-12-dUTP (HPLC, area%). |
| Application: | Fluorescein-12-dUTP is used as a substrate for: • terminal transferase • DNA polymerase I (holoenzyme and Klenow fragment), • Taq DNA polymerase • reverse transcriptase (e.g., from AMV and M-MuLV). Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-d |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Fluorescein-12-dUTP, tetralithium salt. |
| Preparation Note: | The unopened reagent is stable at 15 to 25°C until the expiration date printed on the label. Decomposition of approx. 5% may occur within 6 months. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | ≥85% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 41116100 |