NBT
ROCHE/11383213001 - 4-Nitro blue tetrazolium chloride, solution
Synonym: Nitroblue tetrazolium chloride
Product Type: Chemical
| assay | >95% |
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | Mr 817.7 |
| packaging | pkg of 3 mL (300 mg) |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | hybridization: suitable |
| Northern blotting: suitable | |
| Southern blotting: suitable |
| Application: | • Immunohistocytochemistr • Western blot • Southern blot • Northern blot • Colony and plaque hybridization • In situ hybridization |
| General description: | NBT is a chromogenic and soluble substance. It is a substrate of alkaline phosphatase (AP) and is converted to an indigo/purple dye on its action. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution (100 mg/ml) in 70% dimethylformamide (DMF) (v/v). The color of the NBT solution can vary between yellow and brown depending on the lot used. Note: The color does not impair the quality or the function of the reagent. In some cases a precipitate may occur which is easily brought into solution by briefly warming the substrate at 37 °C. |
| Preparation Note: | Working solution: Preparation of staining solution • Always prepare this solution fresh shortly before use!For all applications except DIG System: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2. • For DIG System applications: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl • Note: Do not include MgCl2 in the DIG detection buffer as this might lead to spotty background on the membrane after the detection procedure. Alkaline Phosphatase does not require Mg2+. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H332 - H360D |
| Precautionary statements | P201 - P261 - P264 - P280 - P304 + P340 + P312 - P308 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | 167.0 °F |
| Flash Point(C) | 75 °C |
| Purity | >95% |
| Storage Temp. | −20°C |
| UNSPSC | 41116100 |