Biotin-16-UTP
ROCHE/11388908910 - pkg of 25 μL (250 nmol; 10mM)
Synonym: Biotin-16-UTP; biotin
Product Type: Chemical
| assay | 97.7% (HPLC) |
| color | colorless |
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | Mr 987.5 (biotin-16-UTP-Li4) |
| packaging | pkg of 25 μL (250 nmol; 10mM) |
| Quality Level | 100  |
| shipped in | dry ice |
| solubility | water: miscible |
| storage temp. | −20°C |
| Application: | Biotin-labeled RNA can be used as a hybridization probe for: • Northern blots • Southern blots • Plaque or colony lifts • RNase protection experiments • In situ hybridization • Microarray hybridization The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate. |
| General description: | For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Biotin-16-UTP, tetralithium salt, 10mM solution |
| Quality: | Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | 97.7% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 41116100 |