Transforming Growth Factor-β1, human (hTGF-β1)
ROCHE/11412272001 - recombinant (CHO cells)
Synonym: hTGF
Product Type: Chemical
assay | >95% (SDS-PAGE) |
biological source | human |
form | solution |
impurities | <0.1 EU/μgtested (LAL test) |
manufacturer/tradename | Roche |
mol wt | 25,000 Da |
packaging | pkg of 1 mL (1 μg) |
potency | <0.1 ng/mL EC50 |
Quality Level | 100 ![]() |
recombinant | expressed in CHO cells |
storage condition | avoid repeated freeze/thaw cycles |
storage temp. | −20°C |
UniProt accession no. | P01137 ![]() ![]() |
Application: | Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling. |
Biochem/physiol Actions: | Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development. |
Biochem/physiol Actions: | Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells. |
General description: | Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques. Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Preparation Note: | Working concentration: 0.01 - 10 ng/ml Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml. Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%. Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C. Note: Avoid repeated freezing and thawing. |
Quality: | Purity: >95% (SDS-PAGE) Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test) (Note: 1 EU corresponds to 0.1 ng) |
Sequence: | Chain Length 2 x 112 AA |
Sequence: | Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1. |
Specificity: | Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells. Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed. |
Unit Definition: | EC50 definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells. |
RIDADR | NONH for all modes of transport |
WGK Germany | nwg |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
Purity | >95% (SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352207 |