Transforming Growth Factor-β1, human (hTGF-β1)
ROCHE/11412272001 - recombinant (CHO cells)
Synonym: hTGF
Product Type: Chemical
TGF-beta Signaling
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
| assay | >95% (SDS-PAGE) |
| biological source | human |
| form | solution |
| impurities | <0.1 EU/μgtested (LAL test) |
| manufacturer/tradename | Roche |
| mol wt | 25,000 Da |
| packaging | pkg of 1 mL (1 μg) |
| potency | <0.1 ng/mL EC50 |
| Quality Level | 100  |
| recombinant | expressed in CHO cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | P01137  |
| Application: | Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling. |
| Biochem/physiol Actions: | Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development. |
| Biochem/physiol Actions: | Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells. |
| General description: | Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques. Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: 0.01 - 10 ng/ml Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml. Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%. Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C. Note: Avoid repeated freezing and thawing. |
| Quality: | Purity: >95% (SDS-PAGE) Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test) (Note: 1 EU corresponds to 0.1 ng) |
| Sequence: | Chain Length 2 x 112 AA |
| Sequence: | Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1. |
| Specificity: | Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells. Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed. |
| Unit Definition: | EC50 definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |