Biotin Protein Labeling Kit
ROCHE/11418165001 - sufficient for 5 labeling reactions, kit of 1 (5 components), suitable for immunoblotting, suitable for ELISA
Synonym: Biotin; protein labeling
Product Type: Chemical
| manufacturer/tradename | Roche |
| packaging | kit of 1 (5 components) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | ELISA: suitable |
| immunoblotting: suitable | |
| usage | sufficient for 5 labeling reactions |
| Analysis Note: | Function tested. |
| Application: | Biotin protein labeling kit has been used to produce biotinylated denatured bovine serum albumin (d-BSA) for coating cadmium telluride (CdTe) quantum dot (QD) conjugates. The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA. |
| General description: | For labeling of proteins with biotin. Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproi |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 5 components. |
| Preparation Note: | Working solution: Preparation of the solutions • Blocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water. • PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water. • Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |
| Components | Blocking Reagent (green screw cap); Phosphate Buffered Saline (PBS) (blue screw cap); D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS); Dimethylsulfoxide (DMSO) (red screw cap); Sephadex G-25 Columns |