Chymotrypsin Sequencing Grade
ROCHE/11418467001 - from bovine pancreas
Synonym: chymotrypsin; protease
Product Type: Chemical
| assay | ≥90% |
| biological source | bovine pancreas |
| form | lyophilized (salt-free) |
| manufacturer/tradename | Roche |
| mol wt | Mr 25 kDa |
| optimum pH | 7.0-9.0 |
| packaging | pkg of 4 × 25 μg |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | ≥70 units/mg protein (at 25 °C, with ATEE as a substrate) |
| storage temp. | 2-8°C |
| technique(s) | protein sequencing: suitable |
| Analysis Note: | Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC. |
| Application: | Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. It is suitable for peptide mapping, fingerprinting, and sequence analysis. |
| Biochem/physiol Actions: | Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate. |
| General description: | Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight. Storage conditions (working solution): 2 to 8 °C A solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C. Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, α2-macroglobulin, α1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF. |
| Preparation Note: | Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl2, pH 7.8). |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% |
| activity | specific activity: ≥70 units/mg protein (at 25 °C, with ATEE as a substrate) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.21.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |