Biotin-16-ddUTP
ROCHE/11427598910 - ≥98% (HPLC), solution, 1 mM, suitable for hybridization
Synonym: Bio-16-ddUTP tetralithium salt; 5-(N-[N-Biotinyl-ε-aminocaproyl-γ-aminobutyryl]-3-aminoallyl)-2′,3′-dideoxyuridine 5′-triphosphate
CAS Number: 188755-42-2
Empirical Formula (Hill Notation): C32H52N7O17P3S
Molecular Weight: 931.78
Linear Formula: C32H52N7O17P3S
Product Type: Chemical
| assay | ≥98% (HPLC) |
| concentration | 1 mM |
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | 955.5 (biotin-16-ddUTP-Li4) |
| packaging | pkg of 25 μL (25 nmol; 1mM) |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | hybridization: suitable |
| Application: | • Biotin-16-ddUTP is used as a substrate for: Terminal Transferase • DNA polymerase I (Holoenzyme and Klenow fragment) • T4 DNA polymerase • Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor). Biotin-16-ddUTP is preferentially used for 3′-end labeling of oligonucleotides with Terminal Transferase, recombinant and labeling DNA. • The biotin-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transfers • Colony and plaque screening • In situ hybridization In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors. The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection. Oligonucleotides are enzymatically labeled at their 3′ end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine- triphosphate (biotin-ddUTP). It has been used in the biotinylation of in vitro transcribed tmRNA during surface plasmon resonance (SPR) technique. It has been used in the standard nick translation reaction for tumor DNA labelling during comparative genomic hybridization. |
| Features and Benefits: | Biotin is bound to dideoxyuridine triphosphate via an amide linkage. |
| General description: | Biotin-16-ddUTP, tetralithium salt; 1mM |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Quality: | Typical analysis: At least 85% Biotin-16-ddUTP (HPLC, area%). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | ≥98% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 41116100 |