Peroxidase (POD), activated
ROCHE/11428861001 - from horseradish
Synonym: POD
Product Type: Chemical
| biological source | horseradish |
| form | lyophilized |
| manufacturer/tradename | Roche |
| optimum pH | 6.0-6.5 |
| packaging | pkg of 8 mg (~40 mg lyophilizate) |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | 800 units/mg protein (at 25 °C with ABTS and H2O2 as substrate (pH 5.0).) |
| storage temp. | 2-8°C |
| Analysis Note: | Isoenzymes: >90% isoenzyme C (HPLC) Purity number: 3.0 - 3.5 (A403/A275) |
| Application: | Peroxidase (POD), activated has been used in terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labeling (TUNEL) staining and immunohistochemistry. |
| Application: | The enzyme can be used without pre-activation for labeling water-soluble substances with reactive and accessible primary amino groups (e.g., peptides or proteins) with peroxidase for use in analytical methods. It is particularly suitable for the coupling of antibodies (Ig, Ig Fab, and Ig F(ab′)2 fragments from rabbit, mouse, sheep, and goat) with peroxidase. The resulting conjugate is optimal for use in immunochemical detection systems, for example, ELISA, immunohistochemistry, and immunoblotting procedures. |
| Biochem/physiol Actions: | Peroxidase (POD) helps in the dehydrogenation of several organic compounds like phenols, aromatic amines, hydroquinones etc. |
| General description: | Donor: hydrogen-peroxide oxidoreductase Reagent for the labeling of water-soluble substances carrying primary amino groups with peroxidase from horseradish (HRP). Capacity: sufficient for approximately 6 mg IgG. |
| General description: | Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as NAD peroxidase, NADP peroxidase, fattyacid peroxidase and a group of non specific enzymes from various sources. It is present in animals, higher plants and other organisms. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Dissolving the lyophilizate in 0.5 ml double-dist. Water produces a peroxidase concentration of 16 mg/ml. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: 800 units/mg protein (at 25 °C with ABTS and H2O2 as substrate (pH 5.0).) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.11.1.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |