DNA, MB-grade
ROCHE/11467140001 - from fish sperm
Synonym: DNA
Product Type: Chemical
biological source | fish (sperm) |
color | light yellowopalescent |
concentration | 100 μg/mL |
form | solution (ready-to-use) |
grade | Molecular Biology |
manufacturer/tradename | Roche |
mol wt | 40-1,000 kDa |
packaging | pkg of 500 mg |
Quality Level | 100 |
solubility | water: miscible |
storage temp. | −20°C |
Application: | The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay. Note: Addition of NaCl, sonication or shearing, and denaturing are not required. |
General description: | DNA, MB-grade is used for the prevention of nonspecific hybridization reactions. |
General description: | DNA, MB-grade, is provided as a sonicated, denatured single-stranded DNA fragment mixture. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Physical form: | Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0 |
Preparation Note: | Working concentration: 100μg/ml This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions. |
Sequence: | Chain Length 120 to 3,000 nucleotides |
RIDADR | NONH for all modes of transport |
WGK Germany | nwg |
Flash Point(F) | No data available |
Flash Point(C) | No data available |
Storage Temp. | −20°C |
UNSPSC | 41106310 |