RNA Molecular Weight Marker I, DIG-labeled
ROCHE/11526529910 - solution, 20 ng/μL, pkg of 200 μL (4 μg)
Product Type: Chemical
concentration | 20 ng/μL |
form | solution |
greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
manufacturer/tradename | Roche |
packaging | pkg of 200 μL (4 μg) |
Quality Level | 100 |
storage temp. | −20°C |
Application: | RNA Molecular Weight Marker I, DIG-labeled, is used as a size standard in northern blot analysis when the DIG System for Nucleic Acid Labeling and Detection is used. The digoxigenin-labeled RNA standard can be detected simultaneously with hybridized digoxigenin-labeled probes during the immunological digoxigenin detection reaction, allowing a length determination of the nonradioactively detected RNA on the blot. Either chemiluminescent (e.g., DIG Luminescent Detection Kit) or colorimetric (e.g., DIG Nucleic Acid Detection Kit) detection may be used. |
Features and Benefits: | DIG RNA molecular weight markers consist of a mixture of in vitro-synthesized digoxigenin-labeled RNA chains of defined length for the molecular-weight determination of RNA species on northern blots. After gel electrophoresis of the appropriate DIG RNA molecular weight marker, under denaturing conditions, and northern transfer onto a nylon or nitrocellulose membrane, the corresponding bands are visible with the DIG immunoassay using the chromogenic substrate NBT/BCIP or the chemiluminescent substrate CSPD. |
General description: | The fragments are prepared by in vitro transcription of linearized plasmids with SP6 or T7 RNA Polymerase in separate reactions. The transcripts are then combined at a ratio that gives bands of uniform intensity when separated by gel electrophoresis. The transcripts are labeled in a photodigoxigenin reaction so that a digoxigenin moiety is present every 200th to 300th nucleotide. This does not result in a visible gel shift. Size Range: 0.3 to 6.9 kb |
General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Preparation Note: | Working concentration: Transfer of 40 ng to 100 ng of the labeled RNA Molecular Weight Marker I per lane or 20 ng to 50 ng of RNA Molecular Weight Marker II or III per lane, depending on the reaction time in the detection step, gives a clearly visible banding pattern. Note: Taking the lane (slot / pouch) size of the agarose gel into account we recommend to load 100 ng ( for RNA marker I) and 50 ng ( for RNA marker II or III) into each slot/ pouch. |
Sequence: | Nine fragments: 310, 438, 575, 1049, 1517, 1821, 2661, 4742, and 6948 bases. |
RIDADR | NONH for all modes of transport |
Flash Point(F) | No data available |
Flash Point(C) | No data available |
Storage Temp. | −20°C |
UNSPSC | 41105335 |