Advanced Search



Cell Death Detection ELISA

ROCHE/11544675001 - sufficient for ≤96 tests, suitable for ELISA, kit of 1 (9 components)

Synonym: ELISA

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11544675001 96 tests
$512.00
1/EA
Add To Favorites

 

manufacturer/tradename Roche
packaging kit of 1 (9 components)
Quality Level 100 
storage temp. 2-8°C
technique(s) ELISA: suitable
usage sufficient for ≤96 tests
Application: Cell Death Detection ELISA has been used to measure Caspase-3 activity, intracellular reactive oxygen species (ROS) and cell apoptosis.
Application: For research use only. Not for use in diagnostic procedures.
Specific determination of mono- and oligonucleosomes in the cytoplasmic fraction of cell lysates.
General description: The Cell Death Detection ELISA serves as photometric enzyme immunoassay for the qualitative and quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after induced cell death. This assay is based on the quantitative sandwich-enzyme immunoassay-principle using mouse monoclonal antibodies directed against DNA and histones, respectively. The antibodies used are not species specific.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Packaging: 1 kit containing 9 components.
Preparation Note: Working solution: Coating solution
Predilute 1 ml Coating buffer conc. with 9 ml double dist. water. Shortly before use, dilute 1 ml anti-histone antibody (reconstituted) with 9 ml Coating buffer.

Washing solution
Warm the Washing buffer concentrate to 15 to 25 °C and dilute 40 ml in 360 ml double distilled water. Mix thoroughly.

Sample solution
Preparation of the sample solution depends on the cell system used and the extent of cell death.
Example: Dilute 25 μl of sample in 225 μl Incubation buffer.

Conjugate solution
Dilute 1 ml Anti-DNA-POD (reconstituted) with 9 ml Incubation buffer.

Substrate solution
Depending on the number of samples tested, dissolve 1, 2, or 3 tablets in 5, 10, or 15 ml Substrate buffer.
Allow to come to 15 to 25 °C before use.
Note: The ABTS solution is light sensitive over extended periods of time.
Storage conditions (working solution): Anti-histone
2 to 8 °C for 2 months
Anti-DNA-POD
2 to 8 °C for 2 months
Coating solution
Prepare immediately before use!
Washing solution
2 to 8 °C for 2 months
Sample solution
2 to 8 °C for 2 months
Conjugate solution
Prepare immediately before use!
Substrate solution
2 to 8 °C for 1 month, protected from light.
Reconstitution: Anti-histone
Reconstitute the lyophilizate in 1 ml double-distilled water for 10 minutes.Mix thoroughly.
Anti-DNA-POD
Reconstitute the lyophilizate in 1 ml double-distilled water for 10 minutes. Mix thoroughly.
Specificity: Anti-histone antibody reacts with the histones H1, H2A, H2B, H3, and H4 of various species (e.g., human, mouse, rat, hamster, cow, opossum, and Xenopus). Anti-DNA-POD antibody binds to ss- and dsDNA. Therefore, the ELISA allows the detection of mono- and oligonucleosomes from various species, and may be applied to measure apoptotic cell death in many different cell systems.
Storage Temp. 2-8°C
UNSPSC 41116158
Components Anti-histone antibody (clone H11–4); Anti-DNA-POD antibody (clone MCA-33); Coating Buffer; Washing Buffer; Incubation Buffer, ready-to-use solution; Substrate Buffer, ready-to-use solution; ABTS Substrate Tablet; Microplate Modules; Adhesive Plate Cover Foils

The following items have been added to your cart:

Choose a favorite list for this item:

Catalog Number Description Price
$

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.


Quick Order

* Required


New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.

800-334-7725
office@krackeler.com


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top