RNase, DNase-free, High Concentration
ROCHE/11579681001 - from bovine pancreas
Synonym: Rnase
Product Type: Chemical
| biological source | bovine pancreas |
| concentration | 10 mg/mL |
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 1 mg (10 mg/ml) |
| Quality Level | 100  |
| specific activity | ≥30 U/mg |
| Application: | High concentration, DNase-free RNase has been used for: • Genomic DNA isolation • Isolation of plasmid DNA • Degradation of RNA for flow cytometry |
| General description: | A heterogeneous mixture of ribonucleases specially prepared to be free of deoxyribonuclease activity according to the current Quality Control procedures. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Other Notes: | Unlike most RNase preparations, no boiling before use is necessary to remove DNase activity. |
| Preparation Note: | Working concentration: 50 μg/ml is the recommended working concentration of RNase, DNase-free, for the isolation of genomic DNA. Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0 |
| Quality: | Absence of DNase activity tested according to the current Quality Control procedures. |
| Unit Definition: | One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions: A0 to A1 corresponds to the total conversion, A1 being the final absorbance. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ≥30 U/mg |
| UNSPSC | 41105600 |