BM Chemiluminescence ELISA Substrate (POD)
ROCHE/11582950001
Synonym: ELISA substrate
Product Type: Chemical
Sensitivity of BM Chemiluminescence ELISA Substrate (POD). Comparison of photometric detection using ABTS with chemiluminescence detection.
| color | clear colorless |
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 250 mL (for 2500 wells or 1000 tubes) |
| Quality Level | 100  |
| shipped in | wet ice |
| solubility | water: miscible |
| storage condition | (Keep container tightly closed in a dry and well-ventilated place.) |
| storage temp. | 2-8°C |
| suitability | suitable for chemiluminescence |
| technique(s) | ELISA: suitable |
| Application: | The BM Chemiluminescence ELISA Substrate (POD) provides a substrate solution for peroxidase-based (POD, HRP) secondary detection systems with highly sensitive, enhanced chemiluminescence. This reagent has been optimized for ELISA applications to be run on a microplate chemiluminescence reader (96-well format) or tube-format luminometers. |
| Features and Benefits: | • Improved sensitivity • Large dynamic range • Rapid and constant signal Contents Substrate Reagent A (buffered solution that contains luminol/4-iodophenol) Starting Reagent B (buffered solution that contains a stabilized form of H2O2) |
| General description: | The sensitivity of enzyme-linked immunosorbent assays often depends on the detection limit of the colorimetric substrates used. In addition, the dynamic range of a colorimetric assay is restricted to a maximum of two orders of magnitude due to the physical/chemical limitations of absorbance measurement (Law of Lambert & Beer). With chemiluminescence technology, a nonradioactive method, which combines the convenience of tube or microplate-based immunoassays with the advantages of isotopic assays, is now available. The BM Chemiluminescence ELISA Substrate (POD) has been evaluated with different immunoassays on the LB 96 P microplate reader and the LB 953 tube chemiluminescence analyzer from EG&G;Berthold. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working solution: Preparation of working solution The BM Chemiluminescence ELISA Substrate is supplied as a set of two stable solutions. Depending on the scale of the assay, the appropriate amount of substrate solution has to be prepared 15 minutes before use: • Add to 100 parts of solution A one part of solution B. • Stir the mixture for at least 15 minutes at 15 to 25 °C to equilibrate the components. Premixed working solution stored at 2 to 8 °C should be warmed up to 15 to 25 °C to avoid temperature effects during measurement. Storage conditions (working solution): The premixed solution is stable for 1 week, when stored at 2 to 8 °C. |
| Principle: | Horseradish peroxidase (POD, HRP), in the presence of hydrogen peroxide (H2O2), catalyzes the oxidation of diacylhydrazides such as luminol. A reaction product in an excited state is thus formed, then decays to the ground state by emitting light. Strong enhancement of the light emission is achieved by the agent 4-iodophenol (contained), which acts as a radical transmitter between the formed oxygen radical and luminol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |