NBT

ROCHE/11585029001 - crystals, pkg of 5 g

Synonym: Nitrotetrazolium Blue chloride; 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride; 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT

CAS Number: 298-83-9
Empirical Formula (Hill Notation): C₄₀H₃₀Cl₂N₁₀O₆
Molecular Weight: 817.64
MDL Number: MFCD00012159
Linear Formula: C₄₀H₃₀N₁₀O₆ · 2Cl
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-11585029001	5 g	$730.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

description	4-Nitro blue tetrazolium chloride, crystals
form	crystals
InChI	1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key	FSVCQIDHPKZJSO-UHFFFAOYSA-L
manufacturer/tradename	Roche
packaging	pkg of 5 g
Quality Level	100
SMILES string	[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
storage temp.	2-8°C

Analysis Note:	Absorption: NBT has its maximum absorption at 260 nm [in water], and the final product formazan has its maximum absorption at 605 nm [in nitrobenzole].
Application:	• Colony and plaque hybridization • Immunohistocytochemistry • In situ hybridization • Northern blot • Southern blot • Western blot
Application:	NBT has been used in in situ hybridization.
General description:	NBT is soluble in water, aqueous buffers, and dimethylformamide.
General description:	Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology. In immunolabeling technique, 4-Nitro blue tetrazolium (NBT) serves as an oxidant. The hydrolysis of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) by alkaline phosphatase gives rise to leucoindigo, which is then being reduced by NBT to form an insoluble blue BCI. Certain cells reduce NBT, which is a colorless/yellow dye to blue or black formazan crystals. Due to this property, NBT is prefered in redox histochemistry and in biochemical applications. It is formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Preparation Note:	Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.
Quality:	Performance-controlled in the LDH assay.

Symbol	GHS07
Signal word	Warning
Hazard statements	H302 - H315 - H319 - H335
Precautionary statements	P261 - P264 - P280 - P304 + P340 + P312 - P337 + P313 - P403 + P233
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	does not flash
Flash Point(C)	does not flash

Storage Temp.	2-8°C
UNSPSC	41116100

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