Biotin-High Prime
ROCHE/11585649910 - suitable for hybridization, suitable for dot blot, suitable for Northern blotting, suitable for Southern blotting, solution
Synonym: Biotin
Product Type: Chemical
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 100 μg |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | dot blot: suitable |
| hybridization: suitable | |
| Northern blotting: suitable | |
| Southern blotting: suitable | |
| usage | sufficient for 25 labeling reactions (0.01 to 3 ug template DNA per assay) |
| Application: | • Biotin-High Prime is used for the highly efficient random-primed labeling of DNA probes with Biotin-16-dUTP. Biotin-labeled probes are generated at high yield within one hour or after overnight incubation. Biotin-High Prime labeled probes are used in a variety of hybridization techniques: Southern blots • Northern blots • Dot/slot blots • Screening of gene libraries • In situ hybridizations |
| Features and Benefits: | Contents 5x concentrated random primer mix: 1U/μl Klenow polymerase, labeling grade, 1mM dATP, 1mM dCTP, 1mM dGTP, 0.65mM dTTP, 0.35mM Biotin-16-dUTP, in 50% (v/v) glycerol. |
| General description: | Convenient enzyme and nucleotide mixture for rapid random-primed labeling of DNA with Biotin-16-dUTP. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Assay Time: 80 minutes Sample Materials • DNA fragments of at least 100bp, • Linearized plasmids, cosmid or λDNA, • Supercoiled DNA, Or minimal amounts of DNA (10ng), e.g., DNA restriction fragments isolated from gels or in molten agarose can be used. Labeling efficiency: A standard labeling reaction with 1μg template yields 0.8μg biotin-labeled DNA after 1-hour, and 2μg after a 20-hour incubation at +37°C. |
| Specificity: | Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or by heating to 65 °C for 10 minutes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Storage Temp. | −20°C |
| UNSPSC | 41105500 |