Anti-Bromodeoxyuridine-Peroxidase, Fab fragments
ROCHE/11585860001 - from mouse IgG1(clone: BMG6H8)
Synonym: anti-BrdU; antibody
Product Type: Chemical
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BMG6H8, monoclonal |
| conjugate | peroxidase conjugate |
| form | lyophilized |
| isotype | IgG1 |
| manufacturer/tradename | Roche |
| packaging | pkg of 15 U |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Analysis Note: | Anti-Bromodeoxyuridine shows 10% cross reaction with iodo-deoxyuridine, but no cross reaction to fluoro-deoxyuridine. No cross reaction to any endogenous thymidine or uridine. Cross reactivity with 5-Br-UTP has not been tested but it is suggested that there is a good chance for reaction, because the only difference is an absent hydroxyl group on the ribose distal to the bromine substitution. |
| Application: | Anti-bromodeoxyuridine antibody is suitable for monitoring proliferating cells in blood, tissues, and tumors, as well as for determining BrdU incorporation on a single-cell level using: • ELISA (600 tests) • Immunohistocytochemistr • Cryosections • Paraffin sections |
| General description: | Fab fragments from monoclonal antibody to 5-bromo-2′-deoxyuridine-5 |
| Immunogen: | The epitope is apparently inside the DNA helix. DNA has to be denatured to ssDNA before antibody efficiently binds to DNA-BrdU. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Lyophilizate, stabilized |
| Preparation Note: | Working concentration: ELISA: 200 mU/ml (approximately 0.2 U/ml); Immunocytochemistry 500 to 1000 mU/ml (1.5 U/ml). The concentrations listed in this section should be taken as a guideline. Working solution: Phosphate buffered saline containing 1% BSA, pH 7.4 |
| Reconstitution: | Add 1 ml double-distilled water to a final concentration of 15 U/ml. |
| Specifications: | Clone: BMG-6H8 Ig Class: mouse IgG1 No. of tests: 600 (ELISA), 150 (Immunochemistry) |
| Specificity: | The antibody reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with cellular DNA that contains incorporated BrdU; it shows no crossreactivity with other cellular components. The antibody may be applied for the qualitative measurement of cell proliferation (BrdU incorporation) on a single-cell level, and for quantitative measurement in a cell population by ELISA. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |