Anti-Digoxigenin-POD (poly), Fab fragments
ROCHE/11633716001 - from sheep
Synonym: anti-digoxigenin; digoxigenin
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | sheep |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | lyophilized |
| isotype | IgG |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 U |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | Anti-Digoxigenin-POD (poly), Fab fragments has been used for the detection of digoxigenin-labeled compounds using: • whole-mount in situ hybridization • fluorescence in situ hybridization • enzyme linked immunosorbent assay (ELISA) • dot blot • Southern blot • western blot • double labeling experiments • lectin blots Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required. Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry. |
| General description: | Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: • Dot blot: 50 to 100 mU/ml • ELISA: 2 to 50 mU/ml • Western blot: 50 to 100 mU/ml • Southern blot: 50 to 100 mU/ml Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5. 1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. |
| Reconstitution: | Add 1 ml double-distilled water to a final concentration of 50 U/ml. |
| Specificity: | The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |