PCR DIG Probe Synthesis Kit
ROCHE/11636090910 - sufficient for 25 reaction (50 μL final reaction volume)
Synonym: DIG system; probe
Product Type: Chemical
PCR DIG Probe Synthesis Kit
| manufacturer/tradename | Roche |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | PCR: suitable |
| usage | sufficient for 25 reaction (50 μL final reaction volume) |
| Application: | Digoxigenin (DIG)-labeled DNA probes produced from the PCR DIG Probe Synthesis Kit is suitable for low-(single)-copy gene detection of rare mRNA in Southern and northern blots. DIG-labeled DNA probes have also been used for labeling of DNA during in situ hybridization. The concentration of the supplied dUTP-nucleotide mix can be adjusted according to probe length. Labeling effectiveness can quickly be determined on an agarose gel. Stripping and reprobing of membranes is possible multiple times following the protocol in the package insert. One PCR labeling reaction (50 μl) will typically yield enough probe for 20 ml hybridization solution. The kit can be used for approximately 25 reactions (50 μl). |
| General description: | The PCR DIG Probe Synthesis Kit contains an alkali-labile digoxigenin (DIG)-11deoxyuridinetriph The PCR DIG Probe Synthesis Kit contains the Expand High Fidelity DNA polymerase mix. This robust enzyme mix with proofreading activity will polymerize probes 40 bp to 5 kb long using 10 pg plasmid DNA and 10 ng genomic DNA as template. The DIG-labeled probes are stable for over one year. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Packaging: | 1 kit containing 6 components. |
| Quality: | Each lot of the PCR DIG Probe Synthesis Kit is function tested in PCR. Amplification products are assayed in genomic Southern blots. Under PCR conditions as described in this package insert, the control reaction generates an amplification product of 442bp. Due to multiple incorporations of DIG-dUTP during the PCR process, the molecular weight of the PCR products is significantly increased compared to the unlabeled PCR product. A specific fragment pattern is detected after hybridization of the PCR product to 10μg human genomic DNA followed by chemiluminescent detection. |
| Hazard statements | H412 |
| Precautionary statements | P273 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41105500 |
| Components | Enzyme Mix, Expand High Fidelity 3.5 U/μl; PCR DIG Probe Synthesis Mix, containing dATP, dCTP, dGTP (2 mM each) 10x concentrated; PCR Buffer with MgCl2 10x concentrated; dNTP Stock Solution, containing dATP, dCTP, dGTP, dTTP (2 mM each), pH 7.0 10x concentrated; Control Template, plasmid DNA in Tris/EDTA buffer, pH 8.0. The 5-kb plasmid contains the cDNA for the human tissue-type plasminogen activator (tPA) 20 pg/μl; Control PCR Primer Mix, containing 50 pmol of each primer, control PCR primer 1 and 2 2 mM each |