Anti-HA-Peroxidase
ROCHE/11667475001 - from mouse IgG2bκ
Synonym: antibody
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| assay | ≥95% (HPLC and SDS-PAGE) |
| biological source | mouse |
| clone | 12CA5, monoclonal |
| concentration | 0.1 mg/mL |
| conjugate | peroxidase conjugate |
| epitope sequence | YPYDVPDYA |
| form | solution |
| isotype | IgG2bκ |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 μg (500 μl) |
| Quality Level | 100  |
| storage temp. | −20°C |
| Application: | • Use Anti-HA-Peroxidase for the immunochemical detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using: • Dot blot • Western blot Use of Anti-HA-Peroxidase eliminates the need for a secondary detection step. Note: For experiments in which sensitivity is not critical, use Anti-HA-Peroxidase (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity. |
| General description: | Anti-HA-Peroxidase is a monoclonal antibody to the HA peptide, conjugated to peroxidase. Anti-HA-Peroxidase recognizes the HA nonapeptide sequence (YPYDVPDYA), derived from the human influenza virus hemagglutinin protein (amino acids 98-106). The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging". |
| Immunogen: | Amino acids 98-106 from the human influenza virus hemagglutinin protein |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Stabilizers: BSA, sucrose, 0.02% thymol Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:• Western blot: 0.1 μg/ml Working solution: 20-fold dilution of Western Blocking Reagent in 1x PBS. |
| Quality: | Each lot of Anti-HA antibody is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation. |
| Specificity: | Anti-HA-Peroxidase recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal). |
| Specificity: | The antibody reacts with the HA epitope, a nonapeptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | ≥95% (HPLC and SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |