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NBT/BCIP Stock Solution

ROCHE/11681451001

Synonym: 5-bromo-4-chloro-3-indoyl phosphate; BCIP

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11681451001 8 mL
$171.00
1/EA
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form solution
manufacturer/tradename Roche
mol wt 817.7
packaging pkg of 8 mL
Quality Level 100 
storage temp. 2-8°C
Application: • The solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, including: Southern blot
• Northern blot
• Western blot
• Colony and plaque lifts
• In situ hybridization
• and for applications in immunohistochemistry and immunocytochemistry.
Note: NBT/BCIP may be used with both nitrocellulose and nylon membranes.
General description: The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N

Evaluation: visually
Other Notes: For life science research only. Not for use in diagnostic procedures.
Physical form: Solution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in 67% (DMSO) (v/v).
Note: The color of the solution can vary from yellowish to light brown.
Preparation Note: Working solution: Preparation of staining solution
Always prepare this solution fresh shortly before use. • For all applications except DIG System: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
• For DIG System applications: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
Note: Do not include MgCl2 in the detection buffer as this may lead to spotty background on the membrane after the detection procedure. Insoluble in water.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) does not flash
Flash Point(C) does not flash
Storage Temp. 2-8°C
UNSPSC 12352204

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