NBT/BCIP Stock Solution
ROCHE/11681451001
Synonym: 5-
Product Type: Chemical
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | 817.7 |
| packaging | pkg of 8 mL |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | • The solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, including: Southern blot • Northern blot • Western blot • Colony and plaque lifts • In situ hybridization • and for applications in immunohistochemistry and immunocytochemistry. Note: NBT/BCIP may be used with both nitrocellulose and nylon membranes. |
| General description: | The reaction product has a blue color and is insoluble in water. The blue color can be visually seen. Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N Evaluation: visually |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indoly Note: The color of the solution can vary from yellowish to light brown. |
| Preparation Note: | Working solution: Preparation of staining solution Always prepare this solution fresh shortly before use. • For all applications except DIG System: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2. • For DIG System applications: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl Note: Do not include MgCl2 in the detection buffer as this may lead to spotty background on the membrane after the detection procedure. Insoluble in water. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |