INT/BCIP Stock Solution
ROCHE/11681460001 - solution, pkg of 3 mL
Synonym: 5-
Product Type: Chemical
form | solution |
manufacturer/tradename | Roche |
mol wt | (INT: Mr = 505.7; BCIP toluidine salt: Mr = 433.6) |
packaging | pkg of 3 mL |
Quality Level | 100 ![]() |
storage temp. | 2-8°C |
Application: | INT/BCIP Stock Solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, that includes: • Southern blot • Western blot • immunohistochemistry and immunocytochemistry It has been used for colorogenic in situ hybridization. |
General description: | BCIP (5-bromo-4-chloro-3-indoy |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Physical form: | Solution of 33 mg/ml INT (2-[4-iodophenyl]-3-[4-ni |
Preparation Note: | Working solution: Preparation of 10 ml Staining Solution Bring the stock solution to 15 to 25 °C until all components are dissolved. Add 75 μl of the stock solution to 10 ml 0.1 M Tris-buffer, pH 9.5, 0.05 M MgCl2, 0.1 M NaCl. Note: Prepare the staining solution shortly before use. Preparation of Additional Solutions Required Blocking solution: Dissolve 0.5 g Blocking Reagent in 100 ml TBS, pH 7.5, by heating to 50 to 60 °C (1 hour). Dissolving the components can be accelerated by sonication or by incubation in a microwave oven. Information Note: The solution remains turbid. |
Principle: | BCIP is the AP-substrate which after dephosphorylation reacts further to give a dark-blue indigo dye as an oxidation product. INT is the oxidant producing a red dye. |
Specifications: | Formulas: INT: C19H13CIN5O2; BCIP: C8H6NO4BrCIP x C7H9N |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
Storage Temp. | 2-8°C |
UNSPSC | 12352204 |