INT/BCIP Stock Solution

ROCHE/11681460001 - solution, pkg of 3 mL

Synonym: 5-bromo-4-chloro-3-indoyl phosphate; BCIP

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-11681460001	3 mL	$195.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

form	solution
manufacturer/tradename	Roche
mol wt	(INT: M_r = 505.7; BCIP toluidine salt: M_r = 433.6)
packaging	pkg of 3 mL
Quality Level	100
storage temp.	2-8°C

Application:	INT/BCIP Stock Solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, that includes: • Southern blot • Western blot • immunohistochemistry and immunocytochemistry It has been used for colorogenic in situ hybridization.
General description:	BCIP (5-bromo-4-chloro-3-indoyl phosphate) is the AP (alkaline phosphatase)-substrate which after dephosphorylation reacts further to give a dark-blue indigo dye as an oxidation product. INT (iodonitrotetrazolium) is the oxidant producing a red dye. The reaction product has a reddish-brown color and is insoluble in water.
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Physical form:	Solution of 33 mg/ml INT (2-[4-iodophenyl]-3-[4-nitrophenyl]-5-phenyltetrazolium chloride) and 33 mg/ml BCIP (5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in DMSO)
Preparation Note:	Working solution: Preparation of 10 ml Staining Solution Bring the stock solution to 15 to 25 °C until all components are dissolved. Add 75 μl of the stock solution to 10 ml 0.1 M Tris-buffer, pH 9.5, 0.05 M MgCl₂, 0.1 M NaCl. *Note:* Prepare the staining solution shortly before use. Preparation of Additional Solutions Required Blocking solution: Dissolve 0.5 g Blocking Reagent in 100 ml TBS, pH 7.5, by heating to 50 to 60 °C (1 hour). Dissolving the components can be accelerated by sonication or by incubation in a microwave oven. *Information Note:* The solution remains turbid.
Principle:	BCIP is the AP-substrate which after dephosphorylation reacts further to give a dark-blue indigo dye as an oxidation product. INT is the oxidant producing a red dye.
Specifications:	Formulas: INT: C₁₉H₁₃CIN₅O₂; BCIP: C₈H₆NO₄BrCIP x C₇H₉N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	does not flash
Flash Point(C)	does not flash

Storage Temp.	2-8°C
UNSPSC	12352204

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