AttoPhos
ROCHE/11681982001 - sufficient for 1,800 multiwell tests (100 ul each), set of (3 components), suitable for PCR
Synonym: fluorimetric substrate
Product Type: Chemical
| color | fluorescent yellow |
| manufacturer/tradename | Roche |
| packaging | set of (3 components) |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | PCR: suitable |
| usage | sufficient for 1,800 multiwell tests (100 ul each) |
| Application: | AttoPhos has been used to detect bound probes by an image capture system. It has also been used as a fluorescence substrate in the study to detect antigens by quantitative immuno-PCR (polymerase chain reaction). |
| General description: | AttoPhos is a highly sensitive fluorimetric substrate for the detection of alkaline phosphatase. With an optimal excitation wavelength of 430–440 nm and the emission maximum at 560 nm, an exceptionally large Stokes Shift is seen. This results in an extremely low intrinsic background signal with a detection limit of 10–19 moles for alkaline phosphatase. In addition, the background fluorescence caused by biological molecules (assay components), cuvettes, or microplates is greatly reduced. With these unique properties, AttoPhos is more sensitive by a factor of 100 than the most commonly used fluorescence substrate 4-methylumbelliferyl-phos |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 set containing 3 components |
| Preparation Note: | Working concentration: AttoPhos Substrate Optimal concentration 0.5–1.5 mM with alkaline phosphatase Working solution: AttoPhos working solution Transfer the entire content of one bottle AttoPhos buffer (bottle 2) into one bottle AttoPhos substrate (bottle 1). The resulting AttoPhos substrate concentration is 1 mM. Calibrator solution Prepare appropriate dilutions of Calibrator stock solution (bottle 3) using AttoPhos buffer solution (bottle 2). Storage conditions (working solution): AttoPhos working solution Stable at 2 to 8 °C for 2 weeks. When stored at -15 to -25 °C, the solution is stable for at least 6 months. Note: To avoid inhomogenity after thawing, mix thoroughly.Calibrator solution Stable at 2 to 8 °C for at least 6 months. |
| Symbol |    GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 + H332 - H315 - H318 - H373 |
| Precautionary statements | P260 - P264 - P280 - P304 + P340 + P312 - P305 + P351 + P338 + P310 - P314 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |
| Components | AttoPhos Substrate; AttoPhos Buffer; Calibration Solution |