Fluorescein RNA Labeling Mix
ROCHE/11685619910 - solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization
Synonym: RNA labeling
Product Type: Chemical
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 40 μL |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | hybridization: suitable |
| Northern blotting: suitable | |
| Southern blotting: suitable | |
| usage | sufficient for 20 reactions |
| Application: | Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as: • in situ hybridization to tissues • Southern blots • northern blots • plaque or colony lifts • RNase protection experiments |
| General description: | Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP). |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | 10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C). |
| Principle: | Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer. |
| Quality: | The mix is function tested in combination with the DIG RNA Labeling Kit. |
| Specificity: | Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Storage Temp. | −20°C |
| UNSPSC | 41105500 |