NBT/BCIP Ready-to-Use Tablets
ROCHE/11697471001 - tablet, pkg of 20 tablets
Synonym: NBT/BCIP
Product Type: Chemical
| form | tablet |
| manufacturer/tradename | Roche |
| mol wt | Mr 433.6 (BCIP toluidine salt) |
| Mr 817.7 (NBT) | |
| packaging | pkg of 20 tablets |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | NBT/BCIP Ready-to-Use tablets has been used in: • in situ hybridization. • western blot • southern blot • northern blot • colony and plaque lifts • immunohistochemistry • immunocytochemistry Note: NBT/BCIP may be used with both nitrocellulose and nylon membranes. |
| General description: | BCIP is the alkaline phosphatase (AP) substrate, which after dephosphorylation is oxidized by NBT to yield a dark-blue indigo precipitating dye. NBT is thereby reduced to a dark-blue precipitating dye and serves to intensify the color reaction making the detection more sensitive. The reaction product has a blue color and is insoluble in water. The blue color can be visually seen. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working solution: Preparation of Color Substrate Solution Add 1 tablet to 10 ml double-dist. water to prepare a ready-to-use staining solution. Composition of staining solution (pH 9.5) after addition of double-dist. water: 0.4 mg/ml NBT; 0.19 mg/ml BCIP; 100 mM Tris buffer, pH 9.5; 50 mM MgSO4 Information Note: The color of the solution can vary from slightly yellowish-violet to light brown. Important Note: Prepare the solution shortly before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |