PCR Buffer Without MgCl2, 10x concentrated
ROCHE/11699105001 - solution, pkg of 3 × 1 mL
Synonym: buffer
Product Type: Chemical
The important role of magnesium in PCR.
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 3 × 1 mL |
| Quality Level | 100  |
| storage temp. | −20°C |
| Application: | The PCR buffer without MgCl2 has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR). |
| General description: | The PCR buffer without MgCl2, in combination with the MgCl2 stock solution, is used for the individual adjustment of the Mg2+ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg2+ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl2 stock solution. |
| Other Notes: | For general laboratory use. |
| Physical form: | Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41105600 |